RRC ID 30905
Author Hashimoto M, Aruga J, Hosoya Y, Kanegae Y, Saito I, Mikoshiba K.
Title A neural cell-type-specific expression system using recombinant adenovirus vectors.
Journal Hum. Gene Ther.
Abstract We demonstrated neural cell-type-specific gene expression using an adenovirus vector, which is useful for delivering foreign genes into quiescent neural cells. We produced eight recombinant replication-defective adenoviruses carrying the lacZ reporter gene driven by various promoters, including those of the L7/PCP2 gene (highly restricted expression in cerebellar Purkinje cells), and the myelin basic protein (in oligodendrocytes) gene. We demonstrated in vitro and in vivo promoter-driven, neural cell-type-specific gene expression by recombinant adenoviruses. The genes were transferred into these recombinant adenoviruses and expressed with high levels of efficiency in vitro and in vivo. In primary culture, the recombinant adenoviruses AdexL7-NL-LacZ and AdexMBP-NL-LacZ appeared to be expressed in a Purkinje cell and oligodendrocyte-specific manner. Introduction of 10(8) pfu of these viruses into the adult rat cerebellum by stereotactic injection yielded neural cell-type-specific expression without apparent toxicity to the animals. Thus, adenovirus vectors are useful for cell-type-specific therapeutic uses and in studies requiring neural cell-type-specific gene expression.
Volume 7(2)
Pages 149-58
Published 1996-1-20
DOI 10.1089/hum.1996.7.2-149
PMID 8788166
MeSH Adenoviruses, Human / genetics* Animals Cells, Cultured Cerebellum / cytology Female Gene Expression* Gene Transfer Techniques* Genes, Reporter / genetics Genetic Vectors* Humans Lac Operon / genetics Mice Mice, Inbred ICR Myelin Basic Protein / biosynthesis Myelin Basic Protein / genetics Nerve Tissue Proteins / genetics Oligodendroglia / physiology Purkinje Cells / physiology* Rats Rats, Sprague-Dawley Recombinant Fusion Proteins / biosynthesis
IF 3.855
Times Cited 71
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY MEDICINE, RESEARCH & EXPERIMENTAL
Resource
DNA material pAdex L7 (RDB06009) pAdex L7-NL-Cre (RDB06010) Adex L7-NL-Cre (RDB06011) pAdex MBP-SwaI-SV40pA (RDB06014)