RRC ID 30921
Author Takehana Y, Matsuda M, Myosho T, Suster ML, Kawakami K, Shin-I T, Kohara Y, Kuroki Y, Toyoda A, Fujiyama A, Hamaguchi S, Sakaizumi M, Naruse K.
Title Co-option of Sox3 as the male-determining factor on the Y chromosome in the fish Oryzias dancena.
Journal Nat Commun
Abstract Sex chromosomes harbour a primary sex-determining signal that triggers sexual development of the organism. However, diverse sex chromosome systems have been evolved in vertebrates. Here we use positional cloning to identify the sex-determining locus of a medaka-related fish, Oryzias dancena, and find that the locus on the Y chromosome contains a cis-regulatory element that upregulates neighbouring Sox3 expression in developing gonad. Sex-reversed phenotypes in Sox3(Y) transgenic fish, and Sox3(Y) loss-of-function mutants all point to its critical role in sex determination. Furthermore, we demonstrate that Sox3 initiates testicular differentiation by upregulating expression of downstream Gsdf, which is highly conserved in fish sex differentiation pathways. Our results not only provide strong evidence for the independent recruitment of Sox3 to male determination in distantly related vertebrates, but also provide direct evidence that a novel sex determination pathway has evolved through co-option of a transcriptional regulator potentially interacted with a conserved downstream component.
Volume 5
Pages 4157
Published 2014-6-20
DOI 10.1038/ncomms5157
PII ncomms5157
PMID 24948391
MeSH Animals Animals, Genetically Modified Base Sequence Biological Evolution* Cell Differentiation / physiology Chromosome Walking Chromosomes, Artificial, Bacterial Cloning, Molecular Gene Expression Regulation, Developmental / genetics Gene Expression Regulation, Developmental / physiology* Immunohistochemistry In Situ Hybridization India Male Molecular Sequence Data Mutation / genetics Oryzias / genetics* Oryzias / physiology Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction SOXB1 Transcription Factors / genetics SOXB1 Transcription Factors / physiology* Sequence Analysis, DNA Sex Determination Processes / genetics* Sex Determination Processes / physiology Testis / cytology Testis / growth & development Transforming Growth Factor beta / metabolism Y Chromosome / genetics*
IF 12.353
Times Cited 42
WOS Category GENETICS & HEREDITY
Resource
Medaka RS274 RS275