論文 - 詳細
| RRC ID | 3093 |
|---|---|
| 著者 | Ahn YO, Zheng M, Bevan DR, Esen A, Shiu SH, Benson J, Peng HP, Miller JT, Cheng CL, Poulton JE, Shih MC. |
| タイトル | Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35. |
| ジャーナル | Phytochemistry |
| Abstract |
Catalysing the hydrolysis of terminal beta-galactosyl residues from carbohydrates, galactolipids, and glycoproteins, glycoside hydrolase family 35 (beta-galactosidases; BGALs) are widely distributed in plants and believed to play many key roles, including modification of cell wall components. Completion of the Arabidopsis thaliana genome sequencing project has, for the first time, allowed an examination of the total number, gene structure, and evolutionary patterns of all Family 35 members in a representative (model) angiosperm. Reiterative database searches established a multigene family of 17 members (designated BGAL1-BGAL17). Using these genes as query sequences, BLAST and Hidden Markov Model searches identified BGAL genes among 22 other eukaryotes, whose genomic sequences are known. The Arabidopsis (n=17) and rice (n=15) BGAL families were much larger than those of Chlamydomonas, fungi, and animals (n=0-4), and a lineage-specific expansion of BGAL genes apparently occurred after divergence of the Arabidopsis and rice lineages. All plant BGAL genes, with the exception of Arabidopsis BGAL17 and rice Os 9633.m04334, form a monophyletic group. Arabidopsis BGAL expression levels are much higher in mature leaves, roots, flowers, and siliques but are lower in young seedlings. BGAL8, BGAL11, BGAL13, BGAL14, and BGAL16 are expressed only in flowers. Catalytically active BGAL4 was produced in the E. coli and baculoviral expression systems, purified to electrophoretic homogeneity, and partially characterized. The purified enzyme hydrolyzed p- and o-nitrophenyl-beta-d-galactosides. It also cleaved beta-(1,3)-, beta-(1,4)-, and beta-(1,6)-linked galactobiosides and galactotriosides, showing a marked preference for beta-(1,3)- and beta-(1,4)-linkages. |
| 巻・号 | 68(11) |
| ページ | 1510-20 |
| 公開日 | 2007-6-1 |
| DOI | 10.1016/j.phytochem.2007.03.021 |
| PII | S0031-9422(07)00178-1 |
| PMID | 17466346 |
| MeSH | Arabidopsis / enzymology Arabidopsis / genetics* Arabidopsis Proteins / classification Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Arabidopsis Proteins / physiology Escherichia coli / genetics Evolution, Molecular Genome, Plant* Genomics* Molecular Sequence Data Multigene Family Phylogeny Recombinant Proteins / chemistry Recombinant Proteins / metabolism beta-Galactosidase / classification beta-Galactosidase / genetics* beta-Galactosidase / metabolism beta-Galactosidase / physiology |
| IF | 3.044 |
| 引用数 | 60 |
| WOS 分野 | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda07078 |