RRC ID 30966
Author Suzuki A, Suriyagoda L, Shigeyama T, Tominaga A, Sasaki M, Hiratsuka Y, Yoshinaga A, Arima S, Agarie S, Sakai T, Inada S, Jikumaru Y, Kamiya Y, Uchiumi T, Abe M, Hashiguchi M, Akashi R, Sato S, Kaneko T, Tabata S, Hirsch AM.
Title Lotus japonicus nodulation is photomorphogenetically controlled by sensing the red/far red (R/FR) ratio through jasmonic acid (JA) signaling.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract Light is critical for supplying carbon to the energetically expensive, nitrogen-fixing symbiosis between legumes and rhizobia. Here, we show that phytochrome B (phyB) is part of the monitoring system to detect suboptimal light conditions, which normally suppress Lotus japonicus nodule development after Mesorhizobium loti inoculation. We found that the number of nodules produced by L. japonicus phyB mutants is significantly reduced compared with the number produced of WT Miyakojima MG20. To explore causes other than photoassimilate production, the possibility that local control by the root genotype occurred was investigated by grafting experiments. The results showed that the shoot and not the root genotype is responsible for root nodule formation. To explore systemic control mechanisms exclusive of photoassimilation, we moved WT MG20 plants from white light to conditions that differed in their ratios of low or high red/far red (R/FR) light. In low R/FR light, the number of MG20 root nodules dramatically decreased compared with plants grown in high R/FR, although photoassimilate content was higher for plants grown under low R/FR. Also, the expression of jasmonic acid (JA) -responsive genes decreased in both low R/FR light-grown WT and white light-grown phyB mutant plants, and it correlated with decreased jasmonoyl-isoleucine content in the phyB mutant. Moreover, both infection thread formation and root nodule formation were positively influenced by JA treatment of WT plants grown in low R/FR light and white light-grown phyB mutants. Together, these results indicate that root nodule formation is photomorphogenetically controlled by sensing the R/FR ratio through JA signaling.
Volume 108(40)
Pages 16837-42
Published 2011-10-4
DOI 10.1073/pnas.1105892108
PII 1105892108
PMID 21930895
PMC PMC3189040
MeSH Base Sequence Cyclopentanes / metabolism* DNA Primers / genetics Isoleucine / analogs & derivatives Isoleucine / metabolism Light* Loteae / microbiology Loteae / physiology* Molecular Sequence Data Mutagenesis Mutation / genetics Oxylipins / metabolism* Phytochrome B / genetics Phytochrome B / metabolism Plant Root Nodulation / physiology* Plant Shoots / genetics Reverse Transcriptase Polymerase Chain Reaction Rhizobium / physiology* Sequence Analysis, DNA Signal Transduction / physiology* Symbiosis*
IF 9.504
Times Cited 31
WOS Category PLANT SCIENCES
Resource
Lotus / Glycine L. japonicus EMS mutant