| RRC ID |
3110
|
| Author |
Shinohara H, Matsubayashi Y.
|
| Title |
Functional immobilization of plant receptor-like kinase onto microbeads towards receptor array construction and receptor-based ligand fishing.
|
| Journal |
Plant J
|
| Abstract |
Despite the large number of receptor-like kinases (RLKs) in plants, few of their specific ligands are known. Ligand fishing is one of the most challenging post-genomic technologies. Here, we report a strategy for functional immobilization of plant RLKs on microbeads via covalent linkage. Because of the high density of immobilized RLKs, ligand-receptor interaction can be visualized at high sensitivity using fluorescent-labeled ligands under the confocal laser scanning microscope. Moreover, using a receptor-based affinity chromatography system with RLK microbeads, the ligand of the receptor was directly retrieved at high purity from complex natural samples. Our RLK microbeads and receptor-based ligand fishing approach is a feasible alternative to conventional forward genetics and bioassay-based biochemical purification for identification of novel ligand-receptor pairs in plants.
|
| Volume |
52(1)
|
| Pages |
175-84
|
| Published |
2007-10-1
|
| DOI |
10.1111/j.1365-313X.2007.03204.x
|
| PII |
TPJ3204
|
| PMID |
17662033
|
| MeSH |
Base Sequence
Chromatography, High Pressure Liquid
DNA Primers
Fluorescent Dyes
Ligands
Mass Spectrometry
Microscopy, Confocal
Plant Proteins / metabolism*
Plants / enzymology*
Protein Kinases / metabolism*
|
| IF |
6.141
|
| Times Cited |
13
|
|
WOS Category
|
PLANT SCIENCES
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
rpc00008 |
