論文 - 詳細
| RRC ID | 3110 |
|---|---|
| 著者 | Shinohara H, Matsubayashi Y. |
| タイトル | Functional immobilization of plant receptor-like kinase onto microbeads towards receptor array construction and receptor-based ligand fishing. |
| ジャーナル | Plant J |
| Abstract |
Despite the large number of receptor-like kinases (RLKs) in plants, few of their specific ligands are known. Ligand fishing is one of the most challenging post-genomic technologies. Here, we report a strategy for functional immobilization of plant RLKs on microbeads via covalent linkage. Because of the high density of immobilized RLKs, ligand-receptor interaction can be visualized at high sensitivity using fluorescent-labeled ligands under the confocal laser scanning microscope. Moreover, using a receptor-based affinity chromatography system with RLK microbeads, the ligand of the receptor was directly retrieved at high purity from complex natural samples. Our RLK microbeads and receptor-based ligand fishing approach is a feasible alternative to conventional forward genetics and bioassay-based biochemical purification for identification of novel ligand-receptor pairs in plants. |
| 巻・号 | 52(1) |
| ページ | 175-84 |
| 公開日 | 2007-10-1 |
| DOI | 10.1111/j.1365-313X.2007.03204.x |
| PII | TPJ3204 |
| PMID | 17662033 |
| MeSH | Base Sequence Chromatography, High Pressure Liquid DNA Primers Fluorescent Dyes Ligands Mass Spectrometry Microscopy, Confocal Plant Proteins / metabolism* Plants / enzymology* Protein Kinases / metabolism* |
| IF | 6.141 |
| 引用数 | 13 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00008 |