RRC ID 3117
著者 Miya A, Albert P, Shinya T, Desaki Y, Ichimura K, Shirasu K, Narusaka Y, Kawakami N, Kaku H, Shibuya N.
タイトル CERK1, a LysM receptor kinase, is essential for chitin elicitor signaling in Arabidopsis.
ジャーナル Proc Natl Acad Sci U S A
Abstract Chitin is a major component of fungal cell walls and serves as a microbe-associated molecular pattern (MAMP) for the detection of various potential pathogens in innate immune systems of both plants and animals. We recently showed that chitin elicitor-binding protein (CEBiP), plasma membrane glycoprotein with LysM motifs, functions as a cell surface receptor for chitin elicitor in rice. The predicted structure of CEBiP does not contain any intracellular domains, suggesting that an additional component(s) is required for signaling through the plasma membrane into the cytoplasm. Here, we identified a receptor-like kinase, designated CERK1, which is essential for chitin elicitor signaling in Arabidopsis. The KO mutants for CERK1 completely lost the ability to respond to the chitin elicitor, including MAPK activation, reactive oxygen species generation, and gene expression. Disease resistance of the KO mutant against an incompatible fungus, Alternaria brassicicola, was partly impaired. Complementation with the WT CERK1 gene showed cerk1 mutations were responsible for the mutant phenotypes. CERK1 is a plasma membrane protein containing three LysM motifs in the extracellular domain and an intracellular Ser/Thr kinase domain with autophosphorylation/myelin basic protein kinase activity, suggesting that CERK1 plays a critical role in fungal MAMP perception in plants.
巻・号 104(49)
ページ 19613-8
公開日 2007-12-4
DOI 10.1073/pnas.0705147104
PII 0705147104
PMID 18042724
PMC PMC2148337
MeSH Alternaria* Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis / microbiology Arabidopsis Proteins / analysis Arabidopsis Proteins / genetics Arabidopsis Proteins / physiology* Cell Membrane / enzymology Chitin / metabolism* Gene Expression Genetic Complementation Test Genome, Plant Green Fluorescent Proteins / analysis Green Fluorescent Proteins / genetics Mitogen-Activated Protein Kinases / metabolism Molecular Sequence Data Mutation Plant Diseases / genetics* Plant Diseases / microbiology Protein Serine-Threonine Kinases / analysis Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / physiology* Recombinant Fusion Proteins / analysis Recombinant Fusion Proteins / genetics
IF 9.412
引用数 700
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pst14772