| RRC ID |
31329
|
| 著者 |
Leikoski N, Fewer DP, Jokela J, Alakoski P, Wahlsten M, Sivonen K.
|
| タイトル |
Analysis of an inactive cyanobactin biosynthetic gene cluster leads to discovery of new natural products from strains of the genus Microcystis.
|
| ジャーナル |
PLoS One
|
| Abstract |
Cyanobactins are cyclic peptides assembled through the cleavage and modification of short precursor proteins. An inactive cyanobactin gene cluster has been described from the genome Microcystis aeruginosa NIES843. Here we report the discovery of active counterparts in strains of the genus Microcystis guided by this silent cyanobactin gene cluster. The end products of the gene clusters were structurally diverse cyclic peptides, which we named piricyclamides. Some of the piricyclamides consisted solely of proteinogenic amino acids while others contained disulfide bridges and some were prenylated or geranylated. The piricyclamide gene clusters encoded between 1 and 4 precursor genes. They encoded highly diverse core peptides ranging in length from 7-17 amino acids with just a single conserved amino acid. Heterologous expression of the pir gene cluster from Microcystis aeruginosa PCC7005 in Escherichia coli confirmed that this gene cluster is responsible for the biosynthesis of piricyclamides. Chemical analysis demonstrated that Microcystis strains could produce an array of piricyclamides some of which are geranylated or prenylated. The genetic diversity of piricyclamides in a bloom sample was explored and 19 different piricyclamide precursor genes were found. This study provides evidence for a stunning array of piricyclamides in Microcystis, a worldwide occurring bloom forming cyanobacteria.
|
| 巻・号 |
7(8)
|
| ページ |
e43002
|
| 公開日 |
2012-1-1
|
| DOI |
10.1371/journal.pone.0043002
|
| PII |
PONE-D-12-09614
|
| PMID |
22952627
|
| PMC |
PMC3428304
|
| MeSH |
Amino Acid Sequence
Amino Acids / chemistry
Bacterial Proteins / chemistry*
Bacterial Proteins / genetics*
Computational Biology / methods
Disulfides / chemistry
Escherichia coli / metabolism
Genetic Variation
Microcystis / genetics*
Models, Genetic
Molecular Sequence Data
Multigene Family*
Peptides, Cyclic / chemistry*
Peptides, Cyclic / genetics*
Phylogeny
Ribosomes / metabolism
Sequence Homology, Amino Acid
|
| IF |
2.74
|
| 引用数 |
32
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 藻類 |
NIES-843 |
