RRC ID 31345
Author Yoda S, Yamaguchi J, Mita K, Yamamoto K, Banno Y, Ando T, Daimon T, Fujiwara H.
Title The transcription factor Apontic-like controls diverse colouration pattern in caterpillars.
Journal Nat Commun
Abstract Genetic polymorphisms underlie the convergent and divergent evolution of various phenotypes. Diverse colour patterns on caterpillars, which are ecologically important, are good models for understanding the molecular backgrounds of phenotypic diversity. Here we show that a single evolutionarily conserved gene apontic-like (apt-like) encoding for a putative transcription factor accounts for the silkworm p locus, which causes at least 15 different larval markings involved in branch-like markings and eye-spot formation. The expression of apt-like and melanin synthesis genes are upregulated in association with pigmented areas of marking mutants Striped (p(S)) and normal (+(p)) but not in the non-marking allele plain (p). Functional analyses, ectopic expression, RNAi and TALEN, demonstrate that apt-like causes melanin pigmentation in a cell-autonomous manner. These results suggest that variation in p alleles is caused by the differential expression of the gene apt-like which induces targeted elevation of gene expressions in the melanin synthesis pathway.
Volume 5
Pages 4936
Published 2014-9-18
DOI 10.1038/ncomms5936
PII ncomms5936
PMID 25233442
MeSH Alleles Amino Acid Sequence Animals Base Sequence Body Patterning* Bombyx / embryology* Bombyx / genetics* Chromosome Mapping Cloning, Molecular Color Female Gene Expression Regulation, Developmental Gene Regulatory Networks Genetic Linkage Genetic Techniques Genotype Insect Proteins / metabolism* Larva / genetics Male Melanins / metabolism Microscopy, Confocal Models, Genetic Molecular Sequence Data Phenotype Phylogeny Pigmentation Polymorphism, Genetic* RNA Interference Transcription Factors / metabolism* Transgenes
IF 12.121
Times Cited 20
Silkworms c05 f34 g01 p20 u10 A10