論文 - 詳細
| RRC ID | 31347 |
|---|---|
| 著者 | Nakamura MJ, Hotta K, Oka K. |
| タイトル | Raman spectroscopic imaging of the whole Ciona intestinalis embryo during development. |
| ジャーナル | PLoS One |
| Abstract |
Intracellular composition and the distribution of bio-molecules play central roles in the specification of cell fates and morphogenesis during embryogenesis. Consequently, investigation of changes in the expression and distribution of bio-molecules, especially mRNAs and proteins, is an important challenge in developmental biology. Raman spectroscopic imaging, a non-invasive and label-free technique, allows simultaneous imaging of the intracellular composition and distribution of multiple bio-molecules. In this study, we explored the application of Raman spectroscopic imaging in the whole Ciona intestinalis embryo during development. Analysis of Raman spectra scattered from C. intestinalis embryos revealed a number of localized patterns of high Raman intensity within the embryo. Based on the observed distribution of bio-molecules, we succeeded in identifying the location and structure of differentiated muscle and endoderm within the whole embryo, up to the tailbud stage, in a label-free manner. Furthermore, during cell differentiation, we detected significant differences in cell state between muscle/endoderm daughter cells and daughter cells with other fates that had divided from the same mother cells; this was achieved by focusing on the Raman intensity of single Raman bands at 1002 or 1526 cm(-1), respectively. This study reports the first application of Raman spectroscopic imaging to the study of identifying and characterizing differentiating tissues in a whole chordate embryo. Our results suggest that Raman spectroscopic imaging is a feasible label-free technique for investigating the developmental process of the whole embryo of C. intestinalis. |
| 巻・号 | 8(8) |
| ページ | e71739 |
| 公開日 | 2013-1-1 |
| DOI | 10.1371/journal.pone.0071739 |
| PII | PONE-D-13-12051 |
| PMID | 23977129 |
| PMC | PMC3748111 |
| MeSH | Animals Cell Differentiation Cell Lineage Ciona intestinalis / anatomy & histology* Ciona intestinalis / cytology Ciona intestinalis / embryology* Embryo, Nonmammalian / anatomy & histology* Embryo, Nonmammalian / cytology Endoderm / anatomy & histology Endoderm / cytology Endoderm / embryology Muscles / anatomy & histology Muscles / cytology Muscles / embryology Spectrum Analysis, Raman* Staining and Labeling |
| IF | 2.74 |
| 引用数 | 5 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カタユウレイボヤ・(ニッポンウミシダ) | Wild C. int |