Reference - Detail
|Author||Tsuda M, Seong KH, Aigaki T.|
|Title||POSH, a scaffold protein for JNK signaling, binds to ALG-2 and ALIX in Drosophila.|
Plenty of SH3s (POSH) functions as a scaffold protein for the Jun N-terminal kinase (JNK) signal transduction pathway, which leads to cell death in mammalian cultured cells and Drosophila. Here, we show that POSH forms a complex with Apoptosis-linked gene-2 (ALG-2) and ALG-2-interacting protein (ALIX/AIP1) in a calcium-dependent manner. Overexpression of ALG-2 or ALIX in developing imaginal eye discs resulted in roughened or melanized eyes, respectively. These phenotypes were enhanced by co-overexpression of POSH. We found that overexpression of either gene could induce ectopic JNK activation, suggesting that POSH/ALG-2/ALIX may function together in the regulation of the JNK pathway.
|MeSH||Animals Calcium-Binding Proteins / genetics Calcium-Binding Proteins / metabolism* Carrier Proteins / genetics Carrier Proteins / metabolism* Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism* Drosophila / chemistry Drosophila / growth & development Drosophila / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Eye / anatomy & histology Eye / growth & development Eye / metabolism Gene Expression Regulation, Developmental Immunoprecipitation MAP Kinase Kinase 4 / metabolism Microfilament Proteins / genetics Microfilament Proteins / metabolism* Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Protein Interaction Mapping RNA, Messenger / analysis RNA, Messenger / metabolism|
|WOS Category||BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY|