Reference - Detail
| RRC ID | 31704 |
|---|---|
| Author | Yoshimi K, Kaneko T, Voigt B, Mashimo T. |
| Title | Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. |
| Journal | Nat Commun |
| Abstract |
The bacterial CRISPR/Cas system has proven to be an efficient gene-targeting tool in various organisms. Here we employ CRISPR/Cas for accurate and efficient genome editing in rats. The synthetic chimeric guide RNAs (gRNAs) discriminate a single-nucleotide polymorphism (SNP) difference in rat embryonic fibroblasts, allowing allele-specific genome editing of the dominant phenotype in (F344 × DA)F1 hybrid embryos. Interestingly, the targeted allele, initially assessed by the allele-specific gRNA, is repaired by an interallelic gene conversion between homologous chromosomes. Using single-stranded oligodeoxynucleotides, we recover three recessive phenotypes: the albino phenotype by SNP exchange; the non-agouti phenotype by integration of a 19-bp DNA fragment; and the hooded phenotype by eliminating a 7,098-bp insertional DNA fragment, evolutionary-derived from an endogenous retrovirus. Successful in vivo application of the CRISPR/Cas system confirms its importance as a genetic engineering tool for creating animal models of human diseases and its potential use in gene therapy. |
| Volume | 5 |
| Pages | 4240 |
| Published | 2014-6-26 |
| DOI | 10.1038/ncomms5240 |
| PII | ncomms5240 |
| PMID | 24967838 |
| PMC | PMC4083438 |
| MeSH | Agouti Signaling Protein / genetics Alleles Animals CRISPR-Cas Systems* Genetic Engineering / methods* Genetic Therapy / methods Monophenol Monooxygenase / genetics Phenotype Polymorphism, Single Nucleotide Proto-Oncogene Proteins c-kit / genetics RNA Editing* RNA, Guide, Kinetoplastida* Rats |
| IF | 12.121 |
| Times Cited | 107 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 75 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| DNA material | pT7-gRNA:Tyr^c DR274 (albino) (RDB13010) pT7-gRNA:Tyr^c DR274 (wild) (RDB13011) pT7-gRNA:Asip^a DR274 (RDB13012) pT7-gRNA:kit^h -1 DR274 (RDB13013) pT7-gRNA:kit^h -2-1 DR274 (RDB13014) pT7-gRNA:kit^h -2-2 DR274 (RDB13015). |
| Rats | F344/Stm(strainID=266) DA/Slc(strainID=380) PVG/Seac(strainID=113) |
| Human and Animal Cells | Rat-1(RCB1830) |