RRC ID 31779
Author Nakajima K, Nakajima T, Takase M, Yaoita Y.
Title Generation of albino Xenopus tropicalis using zinc-finger nucleases.
Journal Dev. Growth Differ.
Abstract To generate albino lines of Xenopus tropicalis, we injected fertilized eggs with mRNAs encoding zinc-finger nucleases (ZFNs) targeting the tyrosinase coding region. Surprisingly, vitiligo was observed on the skin of F0 frogs that had been injected with ZFN mRNAs, indicating that both tyrosinase genes in the genome were disrupted in all melanocytes within the vitiligo patches. Mutation analysis using genomic DNA from the skin revealed that two mosaic F0 frogs underwent spatially complex tyrosinase gene mutations. The data implies that the ZFN-induced tyrosinase gene ablations occurred randomly over space and time throughout the entire body, possibly until the young tadpole stage, and that melanocyte precursors lacking functional tyrosinase proliferated and formed vitiligo patches. Several albino X. tropicalis, which are compound heterozygotes for biallelic tyrosinase mutations, were obtained by mating the mosaic F0 frogs. To our knowledge, this is the first report of the albino vertebrates generated by the targeted gene knockout.
Volume 54(9)
Pages 777-84
Published 2012-12
DOI 10.1111/dgd.12006
PMID 23106502
MeSH Albinism / genetics* Albinism / pathology Animals Crosses, Genetic DNA Mutational Analysis Deoxyribonucleases / genetics* Deoxyribonucleases / metabolism Gene Knockout Techniques Gene Transfer Techniques Microinjections Monophenol Monooxygenase / genetics* Mutation / genetics Oligonucleotides / genetics Ovum / metabolism Vitiligo / pathology* Xenopus / genetics* Zinc Fingers / genetics
IF 1.496
Times Cited 23
Xenopus tropicalis Ivory Coast line