論文 - 詳細
| RRC ID | 31881 |
|---|---|
| 著者 | Tone Y, Kawahara M, Kawaguchi D, Ueda H, Nagamune T. |
| タイトル | Death signalobody: inducing conditional cell death in response to a specific antigen. |
| ジャーナル | Hum Gene Ther Methods |
| Abstract |
As the possibility of tumorigenesis and undesirable immune responses in patients cannot be completely excluded in gene and cell therapies, a conditional death switch to eliminate the therapeutic cells would be a valuable tool to enhance the safety of these therapies. A few ligand-receptor conditional death switches have already been developed; however, they cannot be used if patients exhibit side effects upon administration of the ligand. Here we demonstrate a death-inducing chimeric antibody named "death signalobody," in which the antigen-antibody system, having virtually infinite ligand-receptor combinations, is utilized for the activation of death signaling. We designed a death signalobody named "SFas," which has an antifluorescein single-chain variable fragment and the cytoplasmic domain of Fas. SFas efficiently induced conditional apoptosis in murine pro-B Ba/F3 cells in response to fluorescein-conjugated bovine serum albumin. Moreover, SFas was also able to induce antigen-dependent conditional apoptosis in human cancer cell lines. The death signalobody technique will be a valuable tool for the conditional elimination of cells of interest in multiple therapeutic applications. |
| 巻・号 | 24(3) |
| ページ | 141-50 |
| 公開日 | 2013-6-1 |
| DOI | 10.1089/hgtb.2012.147 |
| PMID | 23470213 |
| MeSH | Animals Antigen-Antibody Complex Antigens / immunology Antigens / metabolism Apoptosis* Cell Line Cell Survival Fluorescein / chemistry Fluorescein / metabolism Genetic Vectors / genetics Genetic Vectors / metabolism Humans Mice Recombinant Fusion Proteins / genetics* Recombinant Fusion Proteins / metabolism Retroviridae / genetics Serum Albumin, Bovine / chemistry Serum Albumin, Bovine / metabolism Single-Chain Antibodies / genetics* Single-Chain Antibodies / metabolism Transduction, Genetic fas Receptor / genetics* fas Receptor / metabolism |
| IF | 2.446 |
| 引用数 | 7 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pRx hFAS-i-hCD25 (RDB01702) |