RRC ID 31916
著者 Brummer E, Kurita N, Yoshida S, Nishimura K, Miyaji M.
タイトル Killing of Histoplasma capsulatum by gamma-interferon-activated human monocyte-derived macrophages: evidence for a superoxide anion-dependent mechanism.
ジャーナル J Med Microbiol
Abstract The interaction of human macrophages with the yeast form of the thermally dimorphic fungal pathogen, Histoplasma capsulatum, was studied. Macrophages derived from monocytes by culture in vitro for 3 days ingested H. capsulatum, but were neither fungicidal or fungistatic. In contrast, when monocytes were exposed to human recombinant gamma-interferon (gamma-IFN) during their differentiation into macrophages, those macrophages were able to reduce the number of ingested or adherent cfu of H. capsulatum by 44-75% in 2 h. Activation of macrophages for fungicidal activity by gamma-IFN was dose dependent and 500-1000 units ml were optimal. Antibody to gamma-IFN abrogated the gamma-IFN activation process. Killing of H. capsulatum by activated macrophages in 2-h assays could be inhibited by superoxide dismutase but not by sodium azide.
巻・号 35(1)
ページ 29-34
公開日 1991-7-1
DOI 10.1099/00222615-35-1-29
PMID 1649308
MeSH Cell Adhesion Cell Count Cells, Cultured Dose-Response Relationship, Drug Histoplasma / immunology* Humans Interferon-gamma / immunology Interferon-gamma / pharmacology* Macrophage Activation* Macrophages / immunology Macrophages / microbiology* Monocytes / microbiology Neutralization Tests Recombinant Proteins Superoxide Dismutase / pharmacology Superoxides / pharmacology*
IF 2.156
引用数 27
WOS 分野 MICROBIOLOGY
リソース情報
病原微生物 IFM 41329 IFM 41614