論文 - 詳細
RRC ID | 31916 |
---|---|
著者 | Brummer E, Kurita N, Yoshida S, Nishimura K, Miyaji M. |
タイトル | Killing of Histoplasma capsulatum by gamma-interferon-activated human monocyte-derived macrophages: evidence for a superoxide anion-dependent mechanism. |
ジャーナル | J Med Microbiol |
Abstract |
The interaction of human macrophages with the yeast form of the thermally dimorphic fungal pathogen, Histoplasma capsulatum, was studied. Macrophages derived from monocytes by culture in vitro for 3 days ingested H. capsulatum, but were neither fungicidal or fungistatic. In contrast, when monocytes were exposed to human recombinant gamma-interferon (gamma-IFN) during their differentiation into macrophages, those macrophages were able to reduce the number of ingested or adherent cfu of H. capsulatum by 44-75% in 2 h. Activation of macrophages for fungicidal activity by gamma-IFN was dose dependent and 500-1000 units ml were optimal. Antibody to gamma-IFN abrogated the gamma-IFN activation process. Killing of H. capsulatum by activated macrophages in 2-h assays could be inhibited by superoxide dismutase but not by sodium azide. |
巻・号 | 35(1) |
ページ | 29-34 |
公開日 | 1991-7-1 |
DOI | 10.1099/00222615-35-1-29 |
PMID | 1649308 |
MeSH | Cell Adhesion Cell Count Cells, Cultured Dose-Response Relationship, Drug Histoplasma / immunology* Humans Interferon-gamma / immunology Interferon-gamma / pharmacology* Macrophage Activation* Macrophages / immunology Macrophages / microbiology* Monocytes / microbiology Neutralization Tests Recombinant Proteins Superoxide Dismutase / pharmacology Superoxides / pharmacology* |
IF | 2.156 |
引用数 | 27 |
WOS 分野 | MICROBIOLOGY |
リソース情報 | |
病原微生物 | IFM 41329 IFM 41614 |