| RRC ID |
31955
|
| 著者 |
Park S, Sonn JY, Oh Y, Lim C, Choe J.
|
| タイトル |
SIFamide and SIFamide receptor defines a novel neuropeptide signaling to promote sleep in Drosophila.
|
| ジャーナル |
Mol Cells
|
| Abstract |
SIFamide receptor (SIFR) is a Drosophila G protein-coupled receptor for the neuropeptide SIFamide (SIFa). Although the sequence and spatial expression of SIFa are evolutionarily conserved among insect species, the physiological function of SIFa/SIFR signaling remains elusive. Here, we provide genetic evidence that SIFa and SIFR promote sleep in Drosophila. Either genetic ablation of SIFa-expressing neurons in the pars intercerebralis (PI) or pan-neuronal depletion of SIFa expression shortened baseline sleep and reduced sleep-bout length, suggesting that it caused sleep fragmentation. Consistently, RNA interference- mediated knockdown of SIFR expression caused short sleep phenotypes as observed in SIFa-ablated or depleted flies. Using a panel of neuron-specific Gal4 drivers, we further mapped SIFR effects to subsets of PI neurons. Taken together, these results reveal a novel physiological role of the neuropeptide SIFa/SIFR pathway to regulate sleep through sleep-promoting neural circuits in the PI of adult fly brains.
|
| 巻・号 |
37(4)
|
| ページ |
295-301
|
| 公開日 |
2014-4-1
|
| DOI |
10.14348/molcells.2014.2371
|
| PII |
molcells.2014.2371
|
| PMID |
24658384
|
| PMC |
PMC4012077
|
| MeSH |
Animals
Animals, Genetically Modified
Drosophila*
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
Female
Male
Neurons / physiology*
Neuropeptides / metabolism*
Pituitary Gland, Intermediate / pathology*
RNA, Small Interfering / genetics
Receptors, Neuropeptide / genetics
Receptors, Neuropeptide / metabolism*
Signal Transduction / genetics
Sleep* / genetics
Sleep Deprivation / genetics
Sleep Deprivation / metabolism*
Transcription Factors / genetics
|
| IF |
4.081
|
| 引用数 |
39
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
10823R-1 |
