論文 - 詳細
| RRC ID | 31966 |
|---|---|
| 著者 | Yeom E, Hong ST, Choi KW. |
| タイトル | Crumbs interacts with Xpd for nuclear division control in Drosophila. |
| ジャーナル | Oncogene |
| Abstract |
Crumbs (Crb) family proteins are crucial for cell polarity. Recent studies indicate that they are also involved in growth regulation and cancer. However, it is not well-understood how Crb participates in mitotic processes. Here, we report that Drosophila Crb is critically involved in nuclear division by interacting with Xeroderma pigmentosum D (XPD). A novel gene named galla-1 was identified from a genetic screen for crb modifiers. Galla-1 protein shows homology to MIP18, a subunit of the mitotic spindle-associated MMS19-XPD complex. Loss-of-function galla-1 mutants show abnormal chromosome segregation, defective centrosome positions and branched spindles during nuclear division in early embryos. Embryos with loss-of-function or overexpression of crb show similar mitotic defects and genetic interaction with galla-1. Both Galla-1 and Crb proteins show overlapping localization with spindle microtubules during nuclear division. Galla-1 physically interacts with the intracellular domain of Crb. Interestingly, Galla-1 shows little binding to the Drosophila homolog of XPD, but a related protein Galla-2 binds both Crb and Xpd. Loss-of-function galla-2 mutants show similar mitotic defects as galla-1 and strong genetic interaction with crb. Xpd can form a physical complex with Crb. In imaginal disc, Crb overexpression causes tissue overgrowth as well as DNA damages marked by H2Av phosphorylation. These phenotypes are suppressed by reduction of Xpd. Taken together, this study identifies a novel Crb-Galla-Xpd complex and its function for proper chromosome segregation during nuclear division, implicating a potential link between Crb and Xpd-related genome instability. |
| 巻・号 | 34(21) |
| ページ | 2777-89 |
| 公開日 | 2015-5-21 |
| DOI | 10.1038/onc.2014.202 |
| PII | onc2014202 |
| PMID | 25065591 |
| MeSH | Animals Cell Polarity / physiology Chromosome Segregation / physiology DNA Damage / physiology DNA Helicases / metabolism* Drosophila / metabolism* Drosophila Proteins / metabolism* Membrane Proteins / metabolism* Microtubules / metabolism Mitosis / physiology* Phosphorylation / physiology |
| IF | 7.971 |
| 引用数 | 12 |
| WOS 分野 | GENETICS & HEREDITY ONCOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 30152R-1 30152R-2 |