| RRC ID |
31988
|
| 著者 |
Shigematsu M, Ogawa T, Tanaka W, Takahashi K, Kitamoto HK, Hidaka M, Masaki H.
|
| タイトル |
Evidence for DNA cleavage caused directly by a transfer RNA-targeting toxin.
|
| ジャーナル |
PLoS One
|
| Abstract |
The killer yeast species Pichiaacaciae produces a heteromeric killer protein, PaT, that causes DNA damage and arrests the cell cycle of sensitive Saccharomyces cerevisiae in the S phase. However, the mechanism by which DNA damage occurs remains elusive. A previous study has indicated that Orf2p, a subunit of PaT, specifically cleaves an anticodon loop of an S. cerevisiae transfer RNA (tRNA(Gln)mcm5s2UUG). This finding raised a question about whether the DNA damage is a result of the tRNA cleavage or whether Orf2p directly associates with and cleaves the genomic DNA of sensitive yeast cells. We showed that Orf2p cleaves genomic DNA in addition to cleaving tRNA in vitro. This DNA cleavage requires the same Orf2p residue as that needed for tRNA cleavage, His299. The expression of Orf2p, in which His299 was substituted to alanine, abolished the cell cycle arrest of the host cell. Moreover, the translation impairment induced by tRNA cleavage enabled Orf2p to enter the nucleus, thereby inducing histone phosphorylation.
|
| 巻・号 |
8(9)
|
| ページ |
e75512
|
| 公開日 |
2013-1-1
|
| DOI |
10.1371/journal.pone.0075512
|
| PII |
PONE-D-13-19995
|
| PMID |
24069426
|
| PMC |
PMC3775755
|
| MeSH |
Cell Cycle Checkpoints
Cell Nucleus / metabolism
DNA Cleavage*
DNA Damage
Histones / metabolism
Killer Factors, Yeast / metabolism*
Phosphorylation
Protein Biosynthesis
Protein Transport
RNA, Transfer / genetics*
RNA, Transfer / metabolism*
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae Proteins / chemistry
Saccharomyces cerevisiae Proteins / metabolism
|
| IF |
2.74
|
| 引用数 |
2
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
pGMH10 (RDB01955)
pGML20 (RDB01958). |
