RRC ID 31988
Author Shigematsu M, Ogawa T, Tanaka W, Takahashi K, Kitamoto HK, Hidaka M, Masaki H.
Title Evidence for DNA cleavage caused directly by a transfer RNA-targeting toxin.
Journal PLoS One
Abstract The killer yeast species Pichiaacaciae produces a heteromeric killer protein, PaT, that causes DNA damage and arrests the cell cycle of sensitive Saccharomyces cerevisiae in the S phase. However, the mechanism by which DNA damage occurs remains elusive. A previous study has indicated that Orf2p, a subunit of PaT, specifically cleaves an anticodon loop of an S. cerevisiae transfer RNA (tRNA(Gln)mcm5s2UUG). This finding raised a question about whether the DNA damage is a result of the tRNA cleavage or whether Orf2p directly associates with and cleaves the genomic DNA of sensitive yeast cells. We showed that Orf2p cleaves genomic DNA in addition to cleaving tRNA in vitro. This DNA cleavage requires the same Orf2p residue as that needed for tRNA cleavage, His299. The expression of Orf2p, in which His299 was substituted to alanine, abolished the cell cycle arrest of the host cell. Moreover, the translation impairment induced by tRNA cleavage enabled Orf2p to enter the nucleus, thereby inducing histone phosphorylation.
Volume 8(9)
Pages e75512
Published 2013-1-1
DOI 10.1371/journal.pone.0075512
PII PONE-D-13-19995
PMID 24069426
PMC PMC3775755
MeSH Cell Cycle Checkpoints Cell Nucleus / metabolism DNA Cleavage* DNA Damage Histones / metabolism Killer Factors, Yeast / metabolism* Phosphorylation Protein Biosynthesis Protein Transport RNA, Transfer / genetics* RNA, Transfer / metabolism* Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / chemistry Saccharomyces cerevisiae Proteins / metabolism
IF 2.776
Times Cited 2
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
DNA material pGMH10 (RDB01955) pGML20 (RDB01958).