RRC ID 319
著者 Inomata N, Goto H, Itoh M, Isono K.
タイトル A single-amino-acid change of the gustatory receptor gene, Gr5a, has a major effect on trehalose sensitivity in a natural population of Drosophila melanogaster.
ジャーナル Genetics
Abstract Variation in trehalose sensitivity and nucleotide sequence polymorphism of the Gr5a gene encoding the gustatory receptor to sugar trehalose were investigated in 152 male lines of Drosophila melanogaster collected from a natural population. Among the observed 59 segregating sites, some pairs of sites showed significant linkage disequilibrium. A single SNP, which results in the Ala218Thr amino acid change, was significantly associated with trehalose sensitivity, as previously suggested. Threonine at amino acid position 218 was found to be the ancestral form in D. melanogaster, suggesting that low trehalose sensitivity was an ancestral form with respect to the receptor function. There was large genetic variation in trehalose sensitivity. It was continuously distributed, indicating that trehalose sensitivity measured by the behavioral assay is a quantitative trait. These results suggest that apart from the Gr5a gene, other genetic factors contribute to variation in trehalose sensitivity. Nucleotide diversity (pi) and nucleotide variation (theta) per site were 0.00874 and 0.00590, respectively. Fu and Li's test and the MK test showed no significant departure from the expectation of selective neutrality in the Gr5a gene. However, we rejected selective neutrality by Tajima's test and Fay and Wu's test with the observed level of recombination. We discuss possible causes of the observed pattern of nucleotide variation in the gustatory receptor Gr5a gene.
巻・号 167(4)
ページ 1749-58
公開日 2004-8-1
DOI 10.1534/genetics.104.027045
PII 167/4/1749
PMID 15342513
PMC PMC1471011
MeSH Amino Acid Substitution Analysis of Variance Animals Base Sequence DNA / genetics DNA Primers Drosophila Proteins / genetics* Drosophila melanogaster / genetics* Linkage Disequilibrium Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Receptors, Cell Surface / genetics* Taste / physiology* Trehalose*
IF 4.015
引用数 16
WOS 分野 GENETICS & HEREDITY
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