RRC ID 3204
Author Guo C, Fischhaber PL, Luk-Paszyc MJ, Masuda Y, Zhou J, Kamiya K, Kisker C, Friedberg EC.
Title Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis.
Journal EMBO J
Abstract Pol kappa and Rev1 are members of the Y family of DNA polymerases involved in tolerance to DNA damage by replicative bypass [translesion DNA synthesis (TLS)]. We demonstrate that mouse Rev1 protein physically associates with Pol kappa. We show too that Rev1 interacts independently with Rev7 (a subunit of a TLS polymerase, Pol zeta) and with two other Y-family polymerases, Pol iota and Pol eta. Mouse Pol kappa, Rev7, Pol iota and Pol eta each bind to the same approximately 100 amino acid C-terminal region of Rev1. Furthermore, Rev7 competes directly with Pol kappa for binding to the Rev1 C-terminus. Notwithstanding the physical interaction between Rev1 and Pol kappa, the DNA polymerase activity of each measured by primer extension in vitro is unaffected by the complex, either when extending normal primer-termini, when bypassing a single thymine glycol lesion, or when extending certain mismatched primer termini. Our observations suggest that Rev1 plays a role(s) in mediating protein-protein interactions among DNA polymerases required for TLS. The precise function(s) of these interactions during TLS remains to be determined.
Volume 22(24)
Pages 6621-30
Published 2003-12-15
DOI 10.1093/emboj/cdg626
PMID 14657033
PMC PMC291821
MeSH Animals Base Sequence Binding Sites Cloning, Molecular DNA Damage* DNA Primers DNA Repair* DNA-Directed DNA Polymerase / chemistry DNA-Directed DNA Polymerase / genetics* DNA-Directed DNA Polymerase / metabolism Kinetics Mice Nucleotidyltransferases / chemistry Nucleotidyltransferases / metabolism* Plasmids Recombinant Proteins / chemistry Recombinant Proteins / metabolism Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins / chemistry Saccharomyces cerevisiae Proteins / metabolism*
IF 11.227
Times Cited 270
Prokaryotes E. coli ME9042(BL21(DE3))?