論文 - 詳細
| RRC ID | 3234 |
|---|---|
| 著者 | Gaur R, Varshney U. |
| タイトル | Genetic analysis identifies a function for the queC (ybaX) gene product at an initial step in the queuosine biosynthetic pathway in Escherichia coli. |
| ジャーナル | J Bacteriol |
| Abstract |
Queuosine (Q), one of the most complex modifications occurring at the wobble position of tRNAs with GUN anticodons, is implicated in a number of biological activities, including accuracy of decoding, virulence, and cellular differentiation. Despite these important implications, its biosynthetic pathway has remained unresolved. Earlier, we observed that a naturally occurring strain of Escherichia coli B105 lacked Q modification in the tRNAs. In the present study, we developed a genetic screen to map the defect in E. coli B105 to a single gene, queC (renamed from ybaX), predicted to code for a 231-amino-acid-long protein with a pI of 5.6. As analyzed by mobility of tRNA(Tyr) on acid urea gels and two-dimensional thin-layer chromatography of the modified nucleosides, expression of QueC from a plasmid-borne copy confers a Q+ phenotype to E. coli B105. Further, analyses of tRNA(Tyr) from E. coli JE10651 (queA mutant), its derivative generated by deletion of chromosomal queC (queA deltaqueC), and E. coli JE7325, deficient in converting preQ0 to preQ1, have provided the first genetic evidence for the involvement of QueC at a step leading to production of preQ0, the first known intermediate in the generally accepted pathway that utilizes GTP as the starting molecule. In addition, we discuss the possibilities of collaboration of QueC with other cellular proteins in the production of preQ0. |
| 巻・号 | 187(20) |
| ページ | 6893-901 |
| 公開日 | 2005-10-1 |
| DOI | 10.1128/JB.187.20.6893-6901.2005 |
| PII | 187/20/6893 |
| PMID | 16199558 |
| PMC | PMC1251624 |
| MeSH | Amino Acid Sequence Anticodon / genetics DNA Transposable Elements Escherichia coli / genetics* Escherichia coli / metabolism* Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism* Genetic Complementation Test Genetic Testing / methods Molecular Sequence Data Mutation Nucleoside Q / biosynthesis* Phenotype RNA, Transfer, Tyr / chemistry RNA, Transfer, Tyr / genetics Transduction, Genetic |
| IF | 3.006 |
| 引用数 | 30 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | JE10651 JE7325 |