論文 - 詳細
| RRC ID | 3237 |
|---|---|
| 著者 | Ito M, Baba T, Mori H, Mori H. |
| タイトル | Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. |
| ジャーナル | Metab Eng |
| Abstract |
Escherichia coli is one of the best elucidated organisms. However, about 40% of E. coli genes have not been assigned to their function yet. We analyzed 1440 single gene knock-out mutants using the GN2-MicroPlate, which permits assay of 95 carbon-source utilizations simultaneously. In the knock-out library there are 1044 of so called y-genes with no apparent function. The raw dataset was analyzed and genes were interrelated by the clustering method of the GeneSpring software. In the resulted dendrogram of genes, a group of genes with known and related function tended to be assembled into a cluster. Our clustering method would be useful for functional assignment of so called y-genes with no apparent function, since the resulted dendrogram could connect y-genes to phenotype and function of well-studied genes. |
| 巻・号 | 7(4) |
| ページ | 318-27 |
| 公開日 | 2005-7-1 |
| DOI | 10.1016/j.ymben.2005.06.004 |
| PII | S1096-7176(05)00049-2 |
| PMID | 16095938 |
| MeSH | Escherichia coli / genetics* Gene Deletion* Gene Expression Profiling Gene Expression Regulation, Bacterial / genetics Genes, Bacterial / genetics* Oligonucleotide Array Sequence Analysis / methods Phenotype* Software* |
| IF | 7.263 |
| 引用数 | 31 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | Keio collection |