RRC ID 32380
Author Moritomo H, Yamada K, Kojima Y, Suzuki Y, Tani S, Kinoshita H, Sasaki A, Mikuni S, Kinjo M, Kawamata J.
Title A biphenyl type two-photon fluorescence probe for monitoring the mitochondrial membrane potential.
Journal Cell Struct Funct
Abstract Here we describe the design and synthesis of a bifunctional two-photon fluorescence probe, N,N'-‍dimethyl-4,4'-(biphenyl-2,1-ethenediyl)dipyridinium hexafluorophosphate (BP6). HeLa, Hek293, and Paramecium caudatum cells were stained with BP6. BP6 accumulated on the mitochondria of all three cell types when the mitochondrial membrane potential was high. As the mitochondrial membrane potential decreased following the addition of carbonyl cyanide m-chlorophenyl hydrazine, BP6 moved from the mitochondria to the nucleus in a reversible manner, depending on the mitochondrial membrane potential status. The maximum value of the two-photon absorption cross-section of BP6 is 250 GM (1 GM=1×10(-50) cm(4) s molecules(-1) photon(-1)). This value is 3 and 30 times larger, respectively, than that of the conventional mitochondria selective probes, rhodamine 123 and green fluorescence protein. These results suggest that BP6 should be useful for monitoring mitochondrial membrane potential by two-photon excitation.
Volume 39(2)
Pages 125-33
Published 2014-10-16
DOI 10.1247/csf.14006
PII DN/JST.JSTAGE/csf/14006
PMID 25319070
MeSH Biphenyl Compounds / chemistry* Biphenyl Compounds / metabolism Fluorescent Dyes / chemistry* Fluorescent Dyes / metabolism HEK293 Cells HeLa Cells Humans Membrane Potential, Mitochondrial* Microscopy, Fluorescence Paramecium caudatum / cytology Photons Pyridinium Compounds / chemistry* Pyridinium Compounds / metabolism Spectrometry, Fluorescence Staining and Labeling
IF 3.5
Times Cited 10
WOS Category CELL BIOLOGY
Resource
Paramecium KNZ1207 (PC122021A) RB-1 (PC042001A)