RRC ID 32486
著者 Pyo JH, Park JS, Na HJ, Jeon HJ, Lee SH, Kim JG, Park SY, Jin YW, Kim YS, Yoo MA.
タイトル Functional modification of Drosophila intestinal stem cells by ionizing radiation.
ジャーナル Radiat Res
Abstract Although the diverse effects of ionizing radiation on biological and pathological processes at various levels ranging from molecular to whole body are well studied, the effects on adult stem cells by ionizing radiation remain largely unknown. In this study, we characterized the functional modifications of adult Drosophila midgut intestinal stem cells after ionizing radiation treatment. A dose of 10 Gy of radiation decreased the proliferative capacity of intestinal stem cells. Interestingly, after irradiation at 2 Gy, the intestinal stem cells exhibited increased proliferative activity, misdifferentiation and γH2AvD and 8-oxo-dG levels. In addition, the guts irradiated with 2 Gy showed increased JNK and AKT activities. Furthermore, we showed that 2 Gy of ionizing radiation induced centrosome amplification in intestinal stem cells of adult midguts. Our data gives molecular insights into the effects of ionizing radiation on functional modifications of stem cells. The adult Drosophila midgut intestinal stem cells offer a potentially rich new system for the exploration of the biological effects of ionizing radiation.
巻・号 181(4)
ページ 376-86
公開日 2014-4-1
DOI 10.1667/RR13545.1
PMID 24701963
MeSH Animals Cell Proliferation / radiation effects Centrosome DNA Damage Drosophila Intestines / cytology Intestines / radiation effects* Radiation, Ionizing* Stem Cells / cytology Stem Cells / radiation effects*
IF 2.657
引用数 7
WOS 分野 BIOLOGY RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING BIOPHYSICS
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