Reference - Detail
| RRC ID | 32489 |
|---|---|
| Author | Okumura T, Takeda K, Taniguchi K, Adachi-Yamada T. |
| Title | βν integrin inhibits chronic and high level activation of JNK to repress senescence phenotypes in Drosophila adult midgut. |
| Journal | PLoS One |
| Abstract |
Proper control of adult stem cells including their proliferation and differentiation is crucial in maintaining homeostasis of well-organized tissues/organs throughout an organism's life. The Drosophila adult midgut has intestinal stem cells (ISCs), which have been exploited as a simple model system to investigate mechanisms controlling adult tissue homeostasis. Here, we found that a viable mutant of βν integrin (βint-ν), encoding one of two Drosophila integrin β subunits, showed a short midgut and abnormal multilayered epithelia accompanied by an increase in ISC proliferation and misdifferentiation defects. The increase in ISC proliferation and misdifferentiation was due to frequent ISC duplication expanding a pool of ISCs, which was caused by depression of the Notch signalling, and up-regulation of unpaired (upd), a gene encoding an extracellular ligand in the JAK/STAT signalling pathway. In addition, we observed that abnormally high accumulation of filamentous actin (F-actin) was caused in the βint-ν mutant enterocytes. Furthermore, the defects were rescued by suppressing c-Jun N-terminal kinase (JNK) signalling, which was up-regulated in a manner correlated with the defect levels in the above-mentioned βint-ν mutant phenotype. These symptoms observed in young βint-ν mutant midgut were very similar to those in the aged midgut in wild type. Our results suggested that βint-ν has a novel function for the Drosophila adult midgut homeostasis under normal conditions and provided a new insight into possible age-related diseases caused by latent abnormality of an integrin function. |
| Volume | 9(2) |
| Pages | e89387 |
| Published | 2014-2-20 |
| DOI | 10.1371/journal.pone.0089387 |
| PII | PONE-D-13-35362 |
| PMID | 24586740 |
| PMC | PMC3930726 |
| MeSH | Animals Apoptosis Cell Proliferation Cellular Senescence* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism* Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism Enzyme-Linked Immunosorbent Assay Gene Expression Regulation, Developmental* Immunoenzyme Techniques In Situ Hybridization Integrin beta Chains / genetics Integrin beta Chains / metabolism* Intestinal Mucosa / metabolism Intestines / cytology* JNK Mitogen-Activated Protein Kinases / metabolism* Mutation / genetics Phenotype Phylogeny Stem Cells / cytology* Stem Cells / metabolism |
| IF | 2.74 |
| Times Cited | 12 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Drosophila | |