論文 - 詳細
| RRC ID | 32501 |
|---|---|
| 著者 | Hung RJ, Spaeth CS, Yesilyurt HG, Terman JR. |
| タイトル | SelR reverses Mical-mediated oxidation of actin to regulate F-actin dynamics. |
| ジャーナル | Nat Cell Biol |
| Abstract |
Actin's polymerization properties are markedly altered by oxidation of its conserved Met 44 residue. Mediating this effect is a specific oxidation-reduction (redox) enzyme, Mical, that works with Semaphorin repulsive guidance cues and selectively oxidizes Met 44. We now find that this actin-regulatory process is reversible. Employing a genetic approach, we identified a specific methionine sulfoxide reductase (MsrB) enzyme SelR that opposes Mical redox activity and Semaphorin-Plexin repulsion to direct multiple actin-dependent cellular behaviours in vivo. SelR specifically catalyses the reduction of the R isomer of methionine sulfoxide (methionine-R-sulfoxide) to methionine, and we found that SelR directly reduced Mical-oxidized actin, restoring its normal polymerization properties. These results indicate that Mical oxidizes actin stereospecifically to generate actin Met-44-R-sulfoxide (actin(Met(R)O-44)), and also implicate the interconversion of specific Met/Met(R)O residues as a precise means to modulate protein function. Our results therefore uncover a specific reversible redox actin regulatory system that controls cell and developmental biology. |
| 巻・号 | 15(12) |
| ページ | 1445-54 |
| 公開日 | 2013-12-1 |
| DOI | 10.1038/ncb2871 |
| PII | ncb2871 |
| PMID | 24212093 |
| PMC | PMC4254815 |
| MeSH | 3T3 Cells Actins / chemistry Actins / metabolism* Animals Axons / physiology DNA-Binding Proteins / chemistry DNA-Binding Proteins / metabolism* Drosophila Proteins / chemistry Drosophila Proteins / physiology* Drosophila melanogaster / cytology* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism Female Male Methionine Sulfoxide Reductases / chemistry Methionine Sulfoxide Reductases / physiology* Mice Oxidation-Reduction Phenotype Protein Multimerization Signal Transduction |
| IF | 20.042 |
| 引用数 | 76 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | |