RRC ID 3252
Author Shibata T, Hishida T, Kubota Y, Han YW, Iwasaki H, Shinagawa H.
Title Functional overlap between RecA and MgsA (RarA) in the rescue of stalled replication forks in Escherichia coli.
Journal Genes Cells
Abstract Escherichia coli RecA protein plays a role in DNA homologous recombination, recombination repair, and the rescue of stalled or collapsed replication forks. The mgsA (rarA) gene encodes a highly conserved DNA-dependent ATPase, whose yeast orthologue, MGS1, plays a role in maintaining genomic stability. In this study, we show a functional relationship between mgsA and recA during DNA replication. The mgsA recA double mutant grows more slowly and has lower viability than a recA single mutant, but they are equally sensitive to UV-induced DNA damage. Mutations in mgsA and recA cause lethality in DNA polymerase I deficient cells, and suppress the temperature-dependent growth defect of dnaE486 (Pol III alpha-catalytic subunit). Moreover, recAS25P, a novel recA allele identified in this work, does not complement the slow growth of DeltamgsA DeltarecA cells or the lethality of polA12 DeltarecA, but is proficient in DNA repair, homologous recombination, SOS mutagenesis and SOS induction. These results suggest that RecA and MgsA are functionally redundant in rescuing stalled replication forks, and that the DNA repair and homologous recombination functions of RecA are separated from its function to maintain progression of replication fork.
Volume 10(3)
Pages 181-91
Published 2005-3-1
DOI 10.1111/j.1365-2443.2005.00831.x
PMID 15743409
MeSH Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* DNA Helicases / genetics DNA Helicases / metabolism* DNA Replication / physiology* Escherichia coli / enzymology Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial Mutation Rec A Recombinases / genetics Rec A Recombinases / metabolism*
IF 1.922
Times Cited 27
Prokaryotes E. coli ME8079(AB1157) JW3110(W3110)