RRC ID 32535
Author Wan P, Wang D, Luo J, Chu D, Wang H, Zhang L, Chen J.
Title Guidance receptor promotes the asymmetric distribution of exocyst and recycling endosome during collective cell migration.
Journal Development
Abstract During collective migration, guidance receptors signal downstream to result in a polarized distribution of molecules, including cytoskeletal regulators and guidance receptors themselves, in response to an extracellular gradient of chemotactic factors. However, the underlying mechanism of asymmetry generation in the context of the migration of a group of cells is not well understood. Using border cells in the Drosophila ovary as a model system for collective migration, we found that the receptor tyrosine kinase (RTK) PDGF/VEGF receptor (PVR) is required for a polarized distribution of recycling endosome and exocyst in the leading cells of the border cell cluster. Interestingly, PVR signaled through the small GTPase Rac to positively affect the levels of Rab11-labeled recycling endosomes, probably in an F-actin-dependent manner. Conversely, the exocyst complex component Sec3 was required for the asymmetric localization of RTK activity and F-actin, similar to that previously reported for the function of Rab11. Together, these results suggested a positive-feedback loop in border cells, in which RTKs such as PVR act to induce a higher level of vesicle recycling and tethering activity in the leading cells, which in turn enables RTK activity to be distributed in a more polarized fashion at the front. We also provided evidence that E-cadherin, the major adhesion molecule for border cell migration, is a specific cargo in the Rab11-labeled recycling endosomes and that Sec3 is required for the delivery of the E-cadherin-containing vesicles to the membrane.
Volume 140(23)
Pages 4797-806
Published 2013-12
DOI 10.1242/dev.094979
PII dev.094979
PMID 24198275
MeSH Actins / metabolism Animals Cadherins / metabolism Cell Movement Drosophila Proteins / metabolism Drosophila melanogaster / cytology Drosophila melanogaster / metabolism* Endosomes / metabolism RNA Interference RNA, Small Interfering Receptors, Platelet-Derived Growth Factor / metabolism* Receptors, Vascular Endothelial Growth Factor / metabolism* Signal Transduction Vesicular Transport Proteins / metabolism rac GTP-Binding Proteins / metabolism
IF 5.413
Times Cited 10
Drosophila sec3[GT]/TM6B (Chen et al. 2005) Rab11[j2D1] Rab5[k08232] sec3[PBac]/TM6B