RRC ID 32551
Author Lerner DW, McCoy D, Isabella AJ, Mahowald AP, Gerlach GF, Chaudhry TA, Horne-Badovinac S.
Title A Rab10-dependent mechanism for polarized basement membrane secretion during organ morphogenesis.
Journal Dev Cell
Abstract Basement membranes (BMs) are specialized extracellular matrices that are essential for epithelial structure and morphogenesis. However, little is known about how BM proteins are delivered to the basal cell surface or how this process is regulated during development. Here, we identify a mechanism for polarized BM secretion in the Drosophila follicle cells. BM proteins are synthesized in a basal endoplasmic reticulum (ER) compartment from localized mRNAs and are then exported through Tango1-positive ER exit sites to basal Golgi clusters. Next, Crag targets Rab10 to structures in the basal cytoplasm, where it restricts protein delivery to the basal surface. These events occur during egg chamber elongation, a morphogenetic process that depends on follicle cell planar polarity and BM remodeling. Significantly, Tango1 and Rab10 are also planar polarized at the basal epithelial surface. We propose that the spatial control of BM production along two tissue axes promotes exocytic efficiency, BM remodeling, and organ morphogenesis.
Volume 24(2)
Pages 159-68
Published 2013-1-28
DOI 10.1016/j.devcel.2012.12.005
PII S1534-5807(12)00578-3
PMID 23369713
PMC PMC3562474
MeSH Animals Aryl Hydrocarbon Receptor Nuclear Translocator / metabolism* Basement Membrane / metabolism* Cell Polarity Collagen Type IV / metabolism Drosophila / cytology Drosophila / embryology* Drosophila / metabolism Drosophila Proteins / metabolism* Endoplasmic Reticulum / metabolism GTP Phosphohydrolases / metabolism Monomeric GTP-Binding Proteins / metabolism* Morphogenesis Organogenesis* Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / genetics Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / metabolism rab GTP-Binding Proteins / metabolism
IF 10.092
Times Cited 70
Drosophila tj-Gal4 (NP1624 DGRC#104055)