RRC ID 32580
Author Kuroda K, Han H, Tani S, Tanigaki K, Tun T, Furukawa T, Taniguchi Y, Kurooka H, Hamada Y, Toyokuni S, Honjo T.
Title Regulation of marginal zone B cell development by MINT, a suppressor of Notch/RBP-J signaling pathway.
Journal Immunity
Abstract We found that Msx2-interacting nuclear target protein (MINT) competed with the intracellular region of Notch for binding to a DNA binding protein RBP-J and suppressed the transactivation activity of Notch signaling. Although MINT null mutant mice were embryonic lethal, MINT-deficient splenic B cells differentiated about three times more efficiently into marginal zone B cells with a concomitant reduction of follicular B cells. MINT is expressed in a cell-specific manner: high in follicular B cells and low in marginal zone B cells. Since Notch signaling directs differentiation of marginal zone B lymphocytes and suppresses that of follicular B lymphocytes in mouse spleen, the results indicate that high levels of MINT negatively regulate Notch signaling and block differentiation of precursor B cells into marginal zone B cells. MINT may serve as a functional homolog of Drosophila Hairless.
Volume 18(2)
Pages 301-12
Published 2003-2
PII S1074-7613(03)00029-3
PMID 12594956
MeSH Animals B-Lymphocyte Subsets / cytology B-Lymphocyte Subsets / metabolism B-Lymphocytes / cytology* B-Lymphocytes / metabolism* Cell Differentiation DNA-Binding Proteins / metabolism Drosophila Drosophila Proteins Gene Targeting Immunoglobulin J Recombination Signal Sequence-Binding Protein Liver / cytology Liver / embryology Membrane Proteins / metabolism* Mice Mice, Knockout Nuclear Proteins / deficiency Nuclear Proteins / genetics Nuclear Proteins / metabolism* Protein Binding Receptors, Notch Signal Transduction Transcriptional Activation
IF 19.734
Times Cited 160
WOS Category IMMUNOLOGY
Resource
DNA material pEF-BOSneo-Myc-MINT (1-3576)-FLAG (RDB06777) pEF-BOSneo-Myc-MINT deltaRAM7-FLAG (RDB13472) pEF-BOSneo-Myc-MINT deltaN (RDB13473) pEF-BOSneo-Myc-MINT deltaN-NLS (RDB13474) pEF-BOSneo-Myc-MINT deltaN-NLS-deltaRAM7 (RDB13475) pEF-BOSneo-Myc-MINT deltaC (RDB13476) pEF-BOSneo-MINT RAM7-Myc (RDB13477) pEF-BOSneo-MINT RAM7-VP16-Myc (RDB13478).