RRC ID 3266
著者 Douchin V, Bohn C, Bouloc P.
タイトル Down-regulation of porins by a small RNA bypasses the essentiality of the regulated intramembrane proteolysis protease RseP in Escherichia coli.
ジャーナル J. Biol. Chem.
Abstract Adaptation to extracytoplasmic stress in Escherichia coli depends on the activation of sigmaE, normally sequestered by the membrane protein RseA. SigmaE is released in response to stress through the successive RseA cleavage by DegS and the RIP protease RseP. SigmaE and proteases that free it from RseA are essential. We isolated a multicopy suppressor that alleviated RseP and DegS requirement. The suppressor encodes a novel small RNA, RseX. Its activity required the RNA-binding protein Hfq. We used the property that small RNAs are often involved in RNA-RNA interactions to capture RseX putative partners; ompA and ompC mRNA, which encode two major outer membrane proteins, were identified. RseX activity was shown to confer an Hfq-dependent coordinate OmpA and OmpC down-regulation. Because RseP is shown to be no longer essential in a strain lacking OmpA and OmpC, we conclude that RseP, which is required for normal sigmaE activation, prevents toxicity due to the presence of two specific outer membrane proteins that are down-regulated by RseX.
巻・号 281(18)
ページ 12253-9
公開日 2006-5-5
DOI 10.1074/jbc.M600819200
PII M600819200
PMID 16513633
MeSH Bacterial Outer Membrane Proteins / metabolism Bacterial Proteins / metabolism Base Sequence Cell Membrane / metabolism Down-Regulation* Endopeptidases / metabolism Endopeptidases / physiology* Escherichia coli / metabolism* Escherichia coli Proteins / metabolism Escherichia coli Proteins / physiology* Host Factor 1 Protein / metabolism Membrane Proteins / metabolism Membrane Proteins / physiology* Models, Biological Molecular Sequence Data Porins / metabolism* RNA, Messenger / metabolism RNA, Small Cytoplasmic / chemistry* RNA, Small Cytoplasmic / metabolism RNA, Small Cytoplasmic / physiology
IF 4.125
引用数 71
原核生物(大腸菌) ME9062(BW25113) CV-48(MC1061) JW0912 JW0940 JW2203 JW5391 JW3368