| RRC ID |
32670
|
| 著者 |
Yang HC, Mikami Y, Imai T, Taguchi H, Nishimura K, Miyaji M, Branchini ML.
|
| タイトル |
Extrusion of fluorescein diacetate by multidrug-resistant Candida albicans.
|
| ジャーナル |
Mycoses
|
| Abstract |
Over-expression of multidrug efflux transporters causes Candida albicans cells to be resistant to azole antifungal agents. There are several kinds of indicator for multidrug resistance (MDR) phenotype of higher eukaryotic cells. Calcein AM is a prefluorochrome that is known as a substrate for multidrug efflux transporters of mammalian cells. We investigated whether calcein AM was also extruded by the ATP-dependent multidrug transporter (cdr1p) of C. albicans. There was no significant difference in the accumulation of calcein AM between MDR cells and drug-susceptible cells of C. albicans even with sodium azide, suggesting that calcein AM may not be associated with the CDR1-gene-related multidrug efflux system of C. albicans. However, a structurally related prefluorochrome derivative, fluorescein diacetate (FDA), was shown to be extruded by the CDR1 mRNA-overexpressing yeast cells. In comparison with drug-susceptible cells, the resistant cells emitted very weak fluorescence when stained with FDA. Furthermore sodium azide increased the fluorescence of the resistant cells more than 20 times, whereas the fluorescence in the drug-susceptible cells with FDA and sodium azide was three to four times stronger. These results suggested that FDA might be extruded by the CDR1-related multidrug efflux transporter of C. albicans.
|
| 巻・号 |
44(9-10)
|
| ページ |
368-74
|
| 公開日 |
2001-11-1
|
| DOI |
10.1046/j.1439-0507.2001.00666.x
|
| PMID |
11766100
|
| MeSH |
Antifungal Agents / pharmacology*
Azoles / pharmacology
Candida albicans / drug effects*
Candida albicans / genetics
Candida albicans / metabolism
Drug Resistance, Multiple, Fungal*
Flow Cytometry
Fluoresceins / analysis*
Fluorescent Dyes / analysis*
Microbial Sensitivity Tests
Microscopy, Fluorescence
Protein Serine-Threonine Kinases / genetics
Protein Serine-Threonine Kinases / metabolism
Protein-Tyrosine Kinases / genetics
Protein-Tyrosine Kinases / metabolism
Schizosaccharomyces pombe Proteins*
|
| IF |
3.575
|
| 引用数 |
4
|
|
WOS 分野
|
MYCOLOGY
DERMATOLOGY
|
| リソース情報 |
| 病原微生物 |
IFM 40213
IFM 47081
IFM 47085
IFM 47087
IFM 47093
IFM 47109
IFM 47114
IFM 47117
IFM 47118
IFM 47120
IFM 47596
IFM 47597
IFM 47601 |
