RRC ID 32749
Author Hojo M, Takashima S, Kobayashi D, Sumeragi A, Shimada A, Tsukahara T, Yokoi H, Narita T, Jindo T, Kage T, Kitagawa T, Kimura T, Sekimizu K, Miyake A, Setiamarga D, Murakami R, Tsuda S, Ooki S, Kakihara K, Naruse K, Takeda H.
Title Right-elevated expression of charon is regulated by fluid flow in medaka Kupffer's vesicle.
Journal Dev Growth Differ
Abstract Recent studies have revealed that a cilium-generated liquid flow in the node has a crucial role in the establishment of the left-right (LR) axis in the mouse. In fish, Kupffer's vesicle (KV), a teleost-specific spherical organ attached to the tail region, is known to have an equivalent role to the mouse node during LR axis formation. However, at present, there has been no report of an asymmetric gene expressed in KV under the control of fluid flow. Here we report the earliest asymmetric gene in teleost KV, medaka charon, and its regulation. Charon is a member of the Cerberus/DAN family of proteins, first identified in zebrafish. Although zebrafish charon was reported to be symmetrically expressed in KV, medaka charon displays asymmetric expression with more intense expression on the right side. This asymmetric expression was found to be regulated by KV flow because symmetric and up-regulated charon expression was observed in flow-defective embryos with immotile cilia or disrupted KV. Taken together, medaka charon is a reliable gene marker for LR asymmetry in KV and thus, will be useful for the analysis of the early steps downstream of the fluid flow.
Volume 49(5)
Pages 395-405
Published 2007-6-1
DOI 10.1111/j.1440-169X.2007.00937.x
PMID 17547649
MeSH Amino Acid Sequence Animal Structures / embryology Animal Structures / physiology Animals Base Sequence Body Fluids / physiology Body Patterning Cloning, Molecular DNA, Complementary / genetics Fish Proteins / genetics Gene Expression Regulation, Developmental Molecular Sequence Data Mutation Nodal Protein Oligodeoxyribonucleotides, Antisense / genetics Oryzias / embryology* Oryzias / genetics* Oryzias / physiology RNA Interference Sequence Homology, Amino Acid Signal Transduction Transforming Growth Factor beta / genetics
IF 1.723
Times Cited 46
Medaka d-rR/TOKYO (MT837)