RRC ID 33028
Author Sakamoto T, Maebayashi K, Nakagawa Y, Imai H.
Title Deletion of the four phospholipid hydroperoxide glutathione peroxidase genes accelerates aging in Caenorhabditis elegans.
Journal Genes Cells
Abstract The glutathione peroxidase (GPx) family is a major antioxidant enzyme family that catalyzes the reduction of a variety of hydroperoxides. GPxs are divided into selenium- and nonselenium-containing GPxs. Because of their efficient antioxidant activity, which depends on the presence of the amino acid residue selenocysteine, selenium-containing GPxs have been the subject of many studies. However, the physiological roles of the nonselenium GPxs remain unclear. Here, we report that the deletion of phospholipid hydroperoxide glutathione peroxidase (PHGPx) homologues causes accelerated aging that leads to a shortened lifespan in Caenorhabditis elegans. PHGPx is an antioxidant enzyme that directly reduces the phospholipid hydroperoxides generated in biomembranes. The quadruple phgpx mutant gpx-1; gpx-2; gpx-6; gpx-7 developed normally, reached adulthood and reproduced as well as the wild type. However, a lifespan analysis showed that the quadruple phgpx mutant had a short maximum lifespan, with an age-related increase in its mortality rate. The intestine is the primary tissue expressing gpx-1, gpx-2, gpx-6 and gpx-7 in C. elegans, and the expression of gpx-6 is greatly enhanced under starvation conditions. These results suggest that the C. elegans PHGPx homologues have important functions in the regulation of aging, probably by reducing oxidative damage in the intestine.
Volume 19(10)
Pages 778-92
Published 2014-10
DOI 10.1111/gtc.12175
PMID 25200408
MeSH Aging / genetics Aging / metabolism Animals Animals, Genetically Modified Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Gene Deletion* Glutathione Peroxidase / genetics* Glutathione Peroxidase / metabolism Intestinal Mucosa / metabolism Oxidation-Reduction Phospholipid Hydroperoxide Glutathione Peroxidase Phospholipids / metabolism Starvation / metabolism
IF 1.922
Times Cited 4
C.elegans tm2895 tm2100 tm2525 tm2535