論文 - 詳細
| RRC ID | 33116 |
|---|---|
| 著者 | Matsumoto Y, Ishii M, Ishii K, Miyaguchi W, Horie R, Inagaki Y, Hamamoto H, Tatematsu K, Uchino K, Tamura T, Sezutsu H, Sekimizu K. |
| タイトル | Transgenic silkworms expressing human insulin receptors for evaluation of therapeutically active insulin receptor agonists. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
We established a transgenic silkworm strain expressing the human insulin receptor (hIR) using the GAL4/UAS system. Administration of human insulin to transgenic silkworms expressing hIR decreased hemolymph sugar levels and facilitated Akt phosphorylation in the fat body. The decrease in hemolymph sugar levels induced by injection of human insulin in the transgenic silkworms expressing hIR was blocked by co-injection of wortmannin, a phosphoinositide 3-kinase inhibitor. Administration of bovine insulin, an hIR ligand, also effectively decreased sugar levels in the transgenic silkworms. These findings indicate that functional hIRs that respond to human insulin were successfully induced in the transgenic silkworms. We propose that the humanized silkworm expressing hIR is useful for in vivo evaluation of the therapeutic activities of insulin receptor agonists. |
| 巻・号 | 455(3-4) |
| ページ | 159-64 |
| 公開日 | 2014-12-12 |
| DOI | 10.1016/j.bbrc.2014.10.143 |
| PII | S0006-291X(14)01972-X |
| PMID | 25449269 |
| MeSH | Amino Acid Sequence Androstadienes / chemistry Animals Animals, Genetically Modified Antigens, CD / biosynthesis* Bombyx / genetics* Cattle Disease Models, Animal Drug Discovery Glucose / analysis Hemolymph / drug effects* Humans Insulin / chemistry* Ligands Molecular Sequence Data Phosphoinositide-3 Kinase Inhibitors Phosphorylation Receptor, Insulin / agonists* Receptor, Insulin / biosynthesis* Signal Transduction Wortmannin |
| IF | 2.985 |
| 引用数 | 13 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
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| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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