RRC ID |
33144
|
著者 |
Wei L, Li Z, Cheng D, Kusakabe T, Pan M, Duan J, Wang Y, Lu C.
|
タイトル |
RNAi silencing of the SoxE gene suppresses cell proliferation in silkworm BmN4 cells.
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ジャーナル |
Mol Biol Rep
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Abstract |
The transcription factor SoxE is mainly expressed in the gonad and involved in the regulation of gonad development and sex determination in animals. Here, we used the silkworm ovary-derived BmN4-SID1 cell line to survey the roles of the silkworm SoxE protein (BmSoxE) and predict its candidate binding targets. RNAi-mediated silencing of BmSoxE expression suppressed cell proliferation in BmN4-SID1 cells. A further cell cycle analysis revealed that this inhibition of cell proliferation was largely due to cell cycle arrest in G1 phase when BmSoxE expression was blocked in BmN4-SID1 cells. Genome-wide microarray expression analyses demonstrated that the expression levels of a set of genes were significantly altered following BmSoxE RNAi. More than half of these genes contained conserved binding sites for HMG box domain of the Sox proteins and were predicted to be candidate binding targets for BmSoxE. Importantly, some of the candidate targets may be associated with the effect of BmSoxE on cell proliferation. Several candidate target genes showed gonad-specific expression in silkworm larvae. Taken together, these data demonstrate that BmSoxE is required for cell proliferation in silkworm BmN4-SID1 cells and provide valuable information for further investigations of the molecular control exerted by the BmSoxE protein over cell proliferation and gonad development in the silkworm.
|
巻・号 |
41(7)
|
ページ |
4769-81
|
公開日 |
2014-7-1
|
DOI |
10.1007/s11033-014-3348-6
|
PMID |
24723138
|
PMC |
PMC4066180
|
MeSH |
Animals
Bombyx / cytology
Bombyx / genetics*
Bombyx / growth & development
Bombyx / metabolism
Cell Line
Cell Proliferation
Female
G1 Phase Cell Cycle Checkpoints / genetics
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gene Silencing
Insect Proteins / genetics*
Insect Proteins / metabolism
Larva / cytology
Larva / genetics
Larva / growth & development
Larva / metabolism
Ovary / cytology
Ovary / growth & development
Ovary / metabolism*
RNA, Small Interfering / genetics
RNA, Small Interfering / metabolism
SOXE Transcription Factors / antagonists & inhibitors
SOXE Transcription Factors / genetics*
SOXE Transcription Factors / metabolism
|
IF |
1.402
|
引用数 |
3
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
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リソース情報 |
カイコ |
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