RRC ID 33144
著者 Wei L, Li Z, Cheng D, Kusakabe T, Pan M, Duan J, Wang Y, Lu C.
タイトル RNAi silencing of the SoxE gene suppresses cell proliferation in silkworm BmN4 cells.
ジャーナル Mol Biol Rep
Abstract The transcription factor SoxE is mainly expressed in the gonad and involved in the regulation of gonad development and sex determination in animals. Here, we used the silkworm ovary-derived BmN4-SID1 cell line to survey the roles of the silkworm SoxE protein (BmSoxE) and predict its candidate binding targets. RNAi-mediated silencing of BmSoxE expression suppressed cell proliferation in BmN4-SID1 cells. A further cell cycle analysis revealed that this inhibition of cell proliferation was largely due to cell cycle arrest in G1 phase when BmSoxE expression was blocked in BmN4-SID1 cells. Genome-wide microarray expression analyses demonstrated that the expression levels of a set of genes were significantly altered following BmSoxE RNAi. More than half of these genes contained conserved binding sites for HMG box domain of the Sox proteins and were predicted to be candidate binding targets for BmSoxE. Importantly, some of the candidate targets may be associated with the effect of BmSoxE on cell proliferation. Several candidate target genes showed gonad-specific expression in silkworm larvae. Taken together, these data demonstrate that BmSoxE is required for cell proliferation in silkworm BmN4-SID1 cells and provide valuable information for further investigations of the molecular control exerted by the BmSoxE protein over cell proliferation and gonad development in the silkworm.
巻・号 41(7)
ページ 4769-81
公開日 2014-7-1
DOI 10.1007/s11033-014-3348-6
PMID 24723138
PMC PMC4066180
MeSH Animals Bombyx / cytology Bombyx / genetics* Bombyx / growth & development Bombyx / metabolism Cell Line Cell Proliferation Female G1 Phase Cell Cycle Checkpoints / genetics Gene Expression Profiling Gene Expression Regulation, Developmental Gene Silencing Insect Proteins / genetics* Insect Proteins / metabolism Larva / cytology Larva / genetics Larva / growth & development Larva / metabolism Ovary / cytology Ovary / growth & development Ovary / metabolism* RNA, Small Interfering / genetics RNA, Small Interfering / metabolism SOXE Transcription Factors / antagonists & inhibitors SOXE Transcription Factors / genetics* SOXE Transcription Factors / metabolism
IF 1.402
引用数 3
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
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