論文 - 詳細
| RRC ID | 33156 |
|---|---|
| 著者 | Suzuki MG, Ito H, Aoki F. |
| タイトル | Effects of RNAi-mediated knockdown of histone methyltransferases on the sex-specific mRNA expression of Imp in the silkworm Bombyx mori. |
| ジャーナル | Int J Mol Sci |
| Abstract |
Sexual differentiation in Bombyx mori is controlled by sex-specific splicing of Bmdsx, which results in the omission of exons 3 and 4 in a male-specific manner. In B. mori, insulin-like growth factor II mRNA-binding protein (Imp) is a male-specific factor involved in male-specific splicing of Bmdsx. Male-specific Imp mRNA results from the male-specific inclusion of exon 8. To verify the link between histone methylation and alternative RNA processing in Imp, we examined the effects of RNAi-mediated knockdown of several histone methyltransferases on the sex-specific mRNA expression of Imp. As a result, male-specific expression of Imp mRNA was completely abolished when expression of the H3K79 methyltransferase DOT1L was repressed to <10% of that in control males. Chromatin immunoprecipitation-quantitative PCR analysis revealed a higher distribution of H3K79me2 in normal males than in normal females across Imp. RNA polymerase II (RNAP II) processivity assays indicated that RNAi knockdown of DOT1L in males caused a twofold decrease in RNAP II processivity compared to that in control males, with almost equivalent levels to those observed in normal females. Inhibition of RNAP II-mediated elongation in male cells repressed the male-specific splicing of Imp. Our data suggest the possibility that H3K79me2 accumulation along Imp is associated with the male-specific alternative processing of Imp mRNA that results from increased RNAP II processivity. |
| 巻・号 | 15(4) |
| ページ | 6772-96 |
| 公開日 | 2014-4-22 |
| DOI | 10.3390/ijms15046772 |
| PII | ijms15046772 |
| PMID | 24758924 |
| PMC | PMC4013661 |
| MeSH | Alternative Splicing Animals Bombyx / enzymology* Bombyx / growth & development Female Histone Methyltransferases Histone-Lysine N-Methyltransferase / antagonists & inhibitors Histone-Lysine N-Methyltransferase / genetics Histone-Lysine N-Methyltransferase / metabolism* Histones / metabolism Insect Proteins / genetics Insect Proteins / metabolism* Male Methylation Ovum / metabolism Polycomb Repressive Complex 2 / antagonists & inhibitors Polycomb Repressive Complex 2 / genetics Polycomb Repressive Complex 2 / metabolism RNA Interference RNA Polymerase II / metabolism RNA, Messenger / metabolism RNA, Small Interfering / metabolism |
| IF | 4.556 |
| 引用数 | 5 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CHEMISTRY, MULTIDISCIPLINARY |
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| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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