| RRC ID |
33156
|
| 著者 |
Suzuki MG, Ito H, Aoki F.
|
| タイトル |
Effects of RNAi-mediated knockdown of histone methyltransferases on the sex-specific mRNA expression of Imp in the silkworm Bombyx mori.
|
| ジャーナル |
Int J Mol Sci
|
| Abstract |
Sexual differentiation in Bombyx mori is controlled by sex-specific splicing of Bmdsx, which results in the omission of exons 3 and 4 in a male-specific manner. In B. mori, insulin-like growth factor II mRNA-binding protein (Imp) is a male-specific factor involved in male-specific splicing of Bmdsx. Male-specific Imp mRNA results from the male-specific inclusion of exon 8. To verify the link between histone methylation and alternative RNA processing in Imp, we examined the effects of RNAi-mediated knockdown of several histone methyltransferases on the sex-specific mRNA expression of Imp. As a result, male-specific expression of Imp mRNA was completely abolished when expression of the H3K79 methyltransferase DOT1L was repressed to <10% of that in control males. Chromatin immunoprecipitation-quantitative PCR analysis revealed a higher distribution of H3K79me2 in normal males than in normal females across Imp. RNA polymerase II (RNAP II) processivity assays indicated that RNAi knockdown of DOT1L in males caused a twofold decrease in RNAP II processivity compared to that in control males, with almost equivalent levels to those observed in normal females. Inhibition of RNAP II-mediated elongation in male cells repressed the male-specific splicing of Imp. Our data suggest the possibility that H3K79me2 accumulation along Imp is associated with the male-specific alternative processing of Imp mRNA that results from increased RNAP II processivity.
|
| 巻・号 |
15(4)
|
| ページ |
6772-96
|
| 公開日 |
2014-4-22
|
| DOI |
10.3390/ijms15046772
|
| PII |
ijms15046772
|
| PMID |
24758924
|
| PMC |
PMC4013661
|
| MeSH |
Alternative Splicing
Animals
Bombyx / enzymology*
Bombyx / growth & development
Female
Histone Methyltransferases
Histone-Lysine N-Methyltransferase / antagonists & inhibitors
Histone-Lysine N-Methyltransferase / genetics
Histone-Lysine N-Methyltransferase / metabolism*
Histones / metabolism
Insect Proteins / genetics
Insect Proteins / metabolism*
Male
Methylation
Ovum / metabolism
Polycomb Repressive Complex 2 / antagonists & inhibitors
Polycomb Repressive Complex 2 / genetics
Polycomb Repressive Complex 2 / metabolism
RNA Interference
RNA Polymerase II / metabolism
RNA, Messenger / metabolism
RNA, Small Interfering / metabolism
|
| IF |
4.556
|
| 引用数 |
5
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CHEMISTRY, MULTIDISCIPLINARY
|
| リソース情報 |
| カイコ |
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