RRC ID 33194
Author Li M, Guo S, Zhang P, Gong J, Zheng A, Zhang Y, Li H.
Title Vinexin-β deficiency protects against cerebral ischaemia/reperfusion injury by inhibiting neuronal apoptosis.
Journal J Neurochem
Abstract Vinexin-β is an adaptor protein that regulates cell adhesion, cytoskeletal organization and signal transduction. Our previous work showed that Vinexin-β protects against cardiac hypertrophy. However, its function in stroke is largely unknown. In the present study, we observed a significant increase in Vinexin-β expression in both human intracerebral haemorrhage and mouse cerebral ischaemia/reperfusion (I/R) injury model, indicating that Vinexin-β is involved in stroke. Next, using Vinexin-β knockout mice, we further demonstrated that Vinexin-β deficiency significantly protected against cerebral I/R injury, as demonstrated by a dramatic decrease in the infarct volume and an improvement in neurological function. Additionally, immunofluorescence and western blotting showed that the deletion of Vinexin-β attenuated neuronal apoptosis. Mechanically, we found that Akt signalling was up-regulated in the brains of the Vinexin-β knockout mice compared with those of the WT control mice after ischaemic injury. Taken together, our results demonstrate that the deletion of Vinexin-β potently protects against ischaemic injury by inhibiting neuronal apoptosis, and this effect may occur via the up-regulation of Akt signalling. Our findings revealed that Vinexin-β acts as a novel modulator of ischaemic injury, suggesting that Vinexin-β may represent an attractive therapeutic target for the prevention of stroke.
Volume 134(2)
Pages 211-21
Published 2015-7-1
DOI 10.1111/jnc.13110
PMID 25824575
MeSH Adaptor Proteins, Signal Transducing / metabolism* Animals Apoptosis / physiology Blotting, Western Brain Ischemia / metabolism* Brain Ischemia / pathology Fluorescent Antibody Technique Humans In Situ Nick-End Labeling Mice Mice, Knockout Muscle Proteins / metabolism* Neurons / pathology* Rats Rats, Sprague-Dawley Real-Time Polymerase Chain Reaction Reperfusion Injury / metabolism* Reperfusion Injury / pathology
IF 4.066
Times Cited 5
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY NEUROSCIENCES
Resource
Mice RBRC01732