Reference - Detail
| RRC ID | 33203 |
|---|---|
| Author | Gawroński P, Ariyadasa R, Himmelbach A, Poursarebani N, Kilian B, Stein N, Steuernagel B, Hensel G, Kumlehn J, Sehgal SK, Gill BS, Gould P, Hall A, Schnurbusch T. |
| Title | A distorted circadian clock causes early flowering and temperature-dependent variation in spike development in the Eps-3Am mutant of einkorn wheat. |
| Journal | Genetics |
| Abstract |
Viable circadian clocks help organisms to synchronize their development with daily and seasonal changes, thereby providing both evolutionary fitness and advantage from an agricultural perspective. A high-resolution mapping approach combined with mutant analysis revealed a cereal ortholog of Arabidopsis thaliana LUX ARRHYTHMO/PHYTOCLOCK 1 (LUX/PCL1) as a promising candidate for the earliness per se 3 (Eps-3A(m)) locus in einkorn wheat (Triticum monococcum L.). Using delayed fluorescence measurements it was shown that Eps-3A(m) containing einkorn wheat accession KT3-5 had a distorted circadian clock. The hypothesis was subsequently confirmed by performing a time course study on central and output circadian clock genes, which showed arrhythmic transcript patterns in KT3-5 under constant ambient conditions, i.e., constant light and temperature. It was also demonstrated that variation in spikelet number between wild-type and mutants is sensitive to temperature, becoming negligible at 25°. These observations lead us to propose that the distorted clock is causative for both early flowering and variation in spike size and spikelet number, and that having a dysfunctional LUX could have neutral, or even positive, effects in warmer climates. To test the latter hypothesis we ascertained sequence variation of LUX in a range of wheat germplasm. We observed a higher variation in the LUX sequence among accessions coming from the warmer climate and a unique in-frame mutation in early-flowering Chinese T. turgidum cultivar 'Tsing Hua no. 559.' Our results emphasize the importance of the circadian clock in temperate cereals as a promising target for adaptation to new environments. |
| Volume | 196(4) |
| Pages | 1253-61 |
| Published | 2014-4-1 |
| DOI | 10.1534/genetics.113.158444 |
| PII | genetics.113.158444 |
| PMID | 24443443 |
| PMC | PMC3982698 |
| MeSH | Chromosome Mapping Circadian Clocks* DNA Mutational Analysis Flowers / genetics Flowers / growth & development* Genetic Variation Mutation Plant Proteins / genetics* Plant Proteins / metabolism Temperature Triticum / classification Triticum / genetics Triticum / growth & development* |
| IF | 4.015 |
| Times Cited | 41 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Wheat | T. monococcum: KT003-001 KT003-005 |