RRC ID 33227
Author Weng R, Cohen SM.
Title Drosophila miR-124 regulates neuroblast proliferation through its target anachronism.
Journal Development
Abstract MicroRNAs (miRNAs) have been implicated as regulators of central nervous system (CNS) development and function. miR-124 is an evolutionarily ancient, CNS-specific miRNA. On the basis of the evolutionary conservation of its expression in the CNS, miR-124 is expected to have an ancient conserved function. Intriguingly, investigation of miR-124 function using antisense-mediated miRNA depletion has produced divergent and in some cases contradictory findings in a variety of model systems. Here we investigated miR-124 function using a targeted knockout mutant and present evidence for a role during central brain neurogenesis in Drosophila melanogaster. miR-124 activity in the larval neuroblast lineage is required to support normal levels of neuronal progenitor proliferation. We identify anachronism (ana), which encodes a secreted inhibitor of neuroblast proliferation, as a functionally important target of miR-124 acting in the neuroblast lineage. ana has previously been thought to be glial specific in its expression and to act from the cortex glia to control the exit of neuroblasts from quiescence into the proliferative phase that generates the neurons of the adult CNS during larval development. We provide evidence that ana is expressed in miR-124-expressing neuroblast lineages and that ana activity must be limited by the action of miR-124 during neuronal progenitor proliferation. We discuss the possibility that the apparent divergence of function of miR-124 in different model systems might reflect functional divergence through target site evolution.
Volume 139(8)
Pages 1427-34
Published 2012-4
DOI 10.1242/dev.075143
PII dev.075143
PMID 22378639
MeSH Animals Cell Lineage Cell Proliferation Central Nervous System / cytology Crosses, Genetic Drosophila melanogaster Gene Expression Regulation, Developmental* In Situ Hybridization, Fluorescence MicroRNAs / genetics MicroRNAs / metabolism MicroRNAs / physiology* Microscopy, Fluorescence / methods Mutation Neurons / cytology* Stem Cells / cytology Transgenes
IF 5.763
Times Cited 38
WOS Category DEVELOPMENTAL BIOLOGY
Resource
Drosophila