Reference - Detail
| RRC ID | 33260 |
|---|---|
| Author | Fukaya T, Iwakawa HO, Tomari Y. |
| Title | MicroRNAs block assembly of eIF4F translation initiation complex in Drosophila. |
| Journal | Mol Cell |
| Abstract |
miRNAs silence their complementary target mRNAs by translational repression as well as by poly(A) shortening and mRNA decay. In Drosophila, miRNAs are typically incorporated into Argonaute1 (Ago1) to form the effector complex called RNA-induced silencing complex (RISC). Ago1-RISC associates with a scaffold protein GW182, which recruits additional silencing factors. We have previously shown that miRNAs repress translation initiation by blocking formation of the 48S and 80S ribosomal complexes. However, it remains unclear how ribosome recruitment is impeded. Here, we examined the assembly of translation initiation factors on the target mRNA under repression. We show that Ago1-RISC induces dissociation of eIF4A, a DEAD-box RNA helicase, from the target mRNA without affecting 5' cap recognition by eIF4E in a manner independent of GW182. In contrast, direct tethering of GW182 promotes dissociation of both eIF4E and eIF4A. We propose that miRNAs act to block the assembly of the eIF4F complex during translation initiation. |
| Volume | 56(1) |
| Pages | 67-78 |
| Published | 2014-10-2 |
| DOI | 10.1016/j.molcel.2014.09.004 |
| PII | S1097-2765(14)00711-4 |
| PMID | 25280104 |
| MeSH | Animals Argonaute Proteins / metabolism Argonaute Proteins / physiology Cells, Cultured Drosophila Drosophila Proteins / metabolism Drosophila Proteins / physiology Eukaryotic Initiation Factor-4A / metabolism Eukaryotic Initiation Factor-4E / metabolism Eukaryotic Initiation Factor-4F / metabolism* MicroRNAs / genetics MicroRNAs / metabolism MicroRNAs / physiology* Models, Genetic* Peptide Chain Initiation, Translational* RNA, Messenger / metabolism RNA-Induced Silencing Complex / metabolism |
| IF | 15.584 |
| Times Cited | 62 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pENTR-eIF4G (RDB13216) |