論文 - 詳細
| RRC ID | 3329 |
|---|---|
| 著者 | Okamoto S, Tamaru A, Nakajima C, Nishimura K, Tanaka Y, Tokuyama S, Suzuki Y, Ochi K. |
| タイトル | Loss of a conserved 7-methylguanosine modification in 16S rRNA confers low-level streptomycin resistance in bacteria. |
| ジャーナル | Mol Microbiol |
| Abstract |
Streptomycin has been an important drug for the treatment of tuberculosis since its discovery in 1944. But numerous strains of Mycobacterium tuberculosis, the bacterial pathogen that causes tuberculosis, are now streptomycin resistant. Although such resistance is often mediated by mutations within rrs, a 16S rRNA gene or rpsL, which encodes the ribosomal protein S12, these mutations are found in a limited proportion of clinically isolated streptomycin-resistant M. tuberculosis strains. Here we have succeeded in identifying a mutation that confers low-level streptomycin resistance to bacteria, including M. tuberculosis. We found that mutations within the gene gidB confer low-level streptomycin resistance and are an important cause of resistance found in 33% of resistant M. tuberculosis isolates. We further clarified that the gidB gene encodes a conserved 7-methylguanosine (m(7)G) methyltransferase specific for the 16S rRNA, apparently at position G527 located in the so-called 530 loop. Thus, we have identified gidB as a new streptomycin-resistance locus and uncovered a resistance mechanism that is mediated by loss of a conserved m(7)G modification in 16S rRNA. The clinical significance of M. tuberculosis gidB mutation also is noteworthy, as gidB mutations emerge spontaneously at a high frequency of 10(-6) and, once emerged, result in vigorous emergence of high-level streptomycin-resistant mutants at a frequency more than 2000 times greater than that seen in wild-type strains. Further studies on the precise function of GidB may provide a basis for developing strategies to suppress pathogenic bacteria, including M. tuberculosis. |
| 巻・号 | 63(4) |
| ページ | 1096-106 |
| 公開日 | 2007-2-1 |
| DOI | 10.1111/j.1365-2958.2006.05585.x |
| PII | MMI5585 |
| PMID | 17238915 |
| MeSH | Base Sequence Drug Resistance, Bacterial / genetics* Escherichia coli / drug effects Escherichia coli / genetics Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Guanosine / analogs & derivatives* Guanosine / metabolism Methyltransferases / genetics Methyltransferases / metabolism Microbial Sensitivity Tests Molecular Sequence Data Mutation Mycobacterium tuberculosis / drug effects Mycobacterium tuberculosis / genetics Mycobacterium tuberculosis / isolation & purification RNA, Ribosomal, 16S / metabolism* Ribosomal Protein S9 Ribosomal Proteins / genetics Streptomycin / pharmacology* Tuberculosis / microbiology |
| IF | 3.418 |
| 引用数 | 165 |
| WOS 分野 | MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9062(BW25113) |