論文 - 詳細
| RRC ID | 33290 |
|---|---|
| 著者 | Ebina H, Misawa N, Kanemura Y, Koyanagi Y. |
| タイトル | Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus. |
| ジャーナル | Sci Rep |
| Abstract |
Even though highly active anti-retroviral therapy is able to keep HIV-1 replication under control, the virus can lie in a dormant state within the host genome, known as a latent reservoir, and poses a threat to re-emerge at any time. However, novel technologies aimed at disrupting HIV-1 provirus may be capable of eradicating viral genomes from infected individuals. In this study, we showed the potential of the CRISPR/Cas9 system to edit the HIV-1 genome and block its expression. When LTR-targeting CRISPR/Cas9 components were transfected into HIV-1 LTR expression-dormant and -inducible T cells, a significant loss of LTR-driven expression was observed after stimulation. Sequence analysis confirmed that this CRISPR/Cas9 system efficiently cleaved and mutated LTR target sites. More importantly, this system was also able to remove internal viral genes from the host cell chromosome. Our results suggest that the CRISPR/Cas9 system may be a useful tool for curing HIV-1 infection. |
| 巻・号 | 3 |
| ページ | 2510 |
| 公開日 | 2013-1-1 |
| DOI | 10.1038/srep02510 |
| PII | srep02510 |
| PMID | 23974631 |
| PMC | PMC3752613 |
| MeSH | CRISPR-Associated Proteins / genetics* CRISPR-Cas Systems* Gene Silencing HEK293 Cells HIV Long Terminal Repeat / genetics* HIV-1 / genetics* HIV-1 / growth & development* HeLa Cells Humans Virus Latency / genetics* |
| IF | 3.998 |
| 引用数 | 287 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 192 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 6.0 |
| リソース情報 | |
| 遺伝子材料 | pgLTR_T5 (RDB12749) pgLTR_T6 (RDB12750) |