RRC ID 33290
Author Ebina H, Misawa N, Kanemura Y, Koyanagi Y.
Title Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus.
Journal Sci Rep
Abstract Even though highly active anti-retroviral therapy is able to keep HIV-1 replication under control, the virus can lie in a dormant state within the host genome, known as a latent reservoir, and poses a threat to re-emerge at any time. However, novel technologies aimed at disrupting HIV-1 provirus may be capable of eradicating viral genomes from infected individuals. In this study, we showed the potential of the CRISPR/Cas9 system to edit the HIV-1 genome and block its expression. When LTR-targeting CRISPR/Cas9 components were transfected into HIV-1 LTR expression-dormant and -inducible T cells, a significant loss of LTR-driven expression was observed after stimulation. Sequence analysis confirmed that this CRISPR/Cas9 system efficiently cleaved and mutated LTR target sites. More importantly, this system was also able to remove internal viral genes from the host cell chromosome. Our results suggest that the CRISPR/Cas9 system may be a useful tool for curing HIV-1 infection.
Volume 3
Pages 2510
Published 2013-1-1
DOI 10.1038/srep02510
PII srep02510
PMID 23974631
PMC PMC3752613
MeSH CRISPR-Associated Proteins / genetics* CRISPR-Cas Systems* Gene Silencing HEK293 Cells HIV Long Terminal Repeat / genetics* HIV-1 / genetics* HIV-1 / growth & development* HeLa Cells Humans Virus Latency / genetics*
IF 3.998
Times Cited 287
DNA material pgLTR_T5 (RDB12749) pgLTR_T6 (RDB12750)