RRC ID 33326
Author Lucena R, Dephoure N, Gygi SP, Kellogg DR, Tallada VA, Daga RR, Jimenez J.
Title Nucleocytoplasmic transport in the midzone membrane domain controls yeast mitotic spindle disassembly.
Journal J. Cell Biol.
Abstract During each cell cycle, the mitotic spindle is efficiently assembled to achieve chromosome segregation and then rapidly disassembled as cells enter cytokinesis. Although much has been learned about assembly, how spindles disassemble at the end of mitosis remains unclear. Here we demonstrate that nucleocytoplasmic transport at the membrane domain surrounding the mitotic spindle midzone, here named the midzone membrane domain (MMD), is essential for spindle disassembly in Schizosaccharomyces pombe cells. We show that, during anaphase B, Imp1-mediated transport of the AAA-ATPase Cdc48 protein at the MMD allows this disassembly factor to localize at the spindle midzone, thereby promoting spindle midzone dissolution. Our findings illustrate how a separate membrane compartment supports spindle disassembly in the closed mitosis of fission yeast.
Volume 209(3)
Pages 387-402
Published 2015-5-11
DOI 10.1083/jcb.201412144
PII jcb.201412144
PMID 25963819
PMC PMC4427787
MeSH Active Transport, Cell Nucleus / physiology Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism Anaphase / physiology* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Nucleus / genetics Cell Nucleus / metabolism* Schizosaccharomyces / cytology Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Spindle Apparatus / genetics Spindle Apparatus / metabolism* Valosin Containing Protein
IF 8.891
Times Cited 1
Yeast S. pombe strains