| RRC ID |
33332
|
| Author |
Hirade Y, Kotoku N, Terasaka K, Saijo-Hamano Y, Fukumoto A, Mizukami H.
|
| Title |
Identification and functional analysis of 2-hydroxyflavanone C-glucosyltransferase in soybean (Glycine max).
|
| Journal |
FEBS Lett
|
| Abstract |
C-Glucosyltransferase is an enzyme that mediates carbon-carbon bond formation to generate C-glucoside metabolites. Although it has been identified in several plant species, the catalytic amino acid residues required for C-glucosylation activity remain obscure. Here, we identified a 2-hydroxyflavanone C-glucosyltransferase (UGT708D1) in soybean. We found that three residues, His20, Asp85, and Arg292, of UGT708D1 were located at the predicted active site and evolutionarily conserved. The substitution of Asp85 or Arg292 with alanine destroyed C-glucosyltransferase activity, whereas the substitution of His20 with alanine abolished C-glucosyltransferase activity but enabled O-glucosyltransferase activity. The catalytic mechanism is discussed on the basis of the findings.
|
| Volume |
589(15)
|
| Pages |
1778-86
|
| Published |
2015-7-8
|
| DOI |
10.1016/j.febslet.2015.05.010
|
| PII |
S0014-5793(15)00363-4
|
| PMID |
25979175
|
| MeSH |
Amino Acid Sequence
Catalysis
Catalytic Domain
Chromatography, High Pressure Liquid
Glucosyltransferases / chemistry
Glucosyltransferases / classification
Glucosyltransferases / metabolism*
Mass Spectrometry
Molecular Sequence Data
Phylogeny
Recombinant Proteins / chemistry
Recombinant Proteins / metabolism
Sequence Homology, Amino Acid
Soybeans / enzymology*
|
| IF |
3.057
|
| Times Cited |
29
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Lotus / Glycine |
GMFL02-51-N04 |
