Reference - Detail
| RRC ID | 33351 |
|---|---|
| Author | Jones KL, Keasling JD. |
| Title | Construction and characterization of F plasmid-based expression vectors. |
| Journal | Biotechnol Bioeng |
| Abstract |
A low-copy expression vector has been constructed from a 9 Kbp region of the Escherichia coli F plasmid containing the oriV and oriS origins of replication. This plasmid carries the beta-lactamase gene (Apr) and the araBAD promoter/araC regulator for arabinose-inducible gene expression. A derivative which carries a lacZ reporter gene was found to be stably maintained for at least 150 generations. A related multi-copy plasmid was stably maintained in arabinose-free medium, but no plasmid-bearing segregants remained after 60 generations when lacZ expression was induced. Induced expression resulted in 27% (multi-copy) and 12% (low-copy) decreases in growth rate. The uninduced levels of beta-galactosidase were 200 units (multi-copy) and 15 units (low-copy). |
| Volume | 59(6) |
| Pages | 659-65 |
| Published | 1998-9-20 |
| PII | 10.1002/(SICI)1097-0290(19980920)59:6<659::AID-BIT1>3.0.CO;2-E |
| PMID | 10099385 |
| MeSH | Arabinose / metabolism Blotting, Southern Cell Division Cloning, Molecular / methods* Escherichia coli / physiology Genetic Vectors / metabolism* Models, Genetic Plasmids / metabolism* Time Factors beta-Galactosidase / metabolism |
| IF | 4.002 |
| Times Cited | 30 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | pKLJ10 pKLJ12 |