RRC ID 33357
著者 Hiromi Y, Okamoto H, Gehring WJ, Hotta Y.
タイトル Germline transformation with Drosophila mutant actin genes induces constitutive expression of heat shock genes.
ジャーナル Cell
Abstract Two Drosophila mutants KM75 and HH5, which are mutated in the act88F actin gene specific for the indirect flight muscles (IFM), synthesize heat shock proteins (hsps) constitutively in a tissue-specific manner. We have introduced cloned mutant act88F genes into a strain containing the wild-type act88F allele by P-element-mediated transformation. Flies transformed with a 4.05 kb KM75 act88F gene fragment encoding the p42 actin variant express both p42 and hsps specifically in the IFM. Using normal/mutant chimeric genes, the mutation sites of KM75 and HH5 were mapped within the sequence encoding the last 72 amino acids of actin. An in vitro mutated gene encoding a protein that lacks the 72 carboxy-terminal amino acids also induces constitutive hsp synthesis.
巻・号 44(2)
ページ 293-301
公開日 1986-1-31
DOI 10.1016/0092-8674(86)90763-4
PII 0092-8674(86)90763-4
PMID 3080247
MeSH Actins / genetics* Animals Base Sequence Drosophila melanogaster / genetics* Flight, Animal Gene Expression Regulation Heat-Shock Proteins / genetics* Molecular Weight Muscles / physiology Mutation Tissue Distribution Transformation, Genetic beta-Galactosidase / genetics
IF 38.637
引用数 86
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
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