論文 - 詳細
RRC ID | 33367 |
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著者 | Penfold RJ, Pemberton JM. |
タイトル | An improved suicide vector for construction of chromosomal insertion mutations in bacteria. |
ジャーナル | Gene |
Abstract |
We have constructed an R6K-based suicide vector (pJP5603) that requires a trans supply of the pir-encoded pi protein of plasmid R6K for replication. Therefore, efficient plasmid suicide results upon transfer to bacteria not harbouring pir. The 3.1-kb vector encodes kanamycin resistance and is mobilizable. When used in conjunction with a JM109 strain carrying pir, it has nine unique restriction sites available for alpha-complementation cloning. Vector functionality was demonstrated in Rhodobacter sphaeroides. |
巻・号 | 118(1) |
ページ | 145-6 |
公開日 | 1992-9-1 |
DOI | 10.1016/0378-1119(92)90263-o |
PII | 0378-1119(92)90263-O |
PMID | 1511879 |
MeSH | Bacterial Proteins / genetics* Chromosomes, Bacterial DNA Helicases* DNA, Recombinant / genetics DNA-Binding Proteins* Genetic Vectors / genetics* Mutagenesis, Insertional / methods* Replicon / genetics Rhodobacter sphaeroides / genetics* Trans-Activators* |
IF | 2.984 |
引用数 | 235 |
WOS 分野 | GENETICS & HEREDITY |
リソース情報 | |
原核生物(大腸菌) | pJP5603 pJP5608 |