RRC ID 33376
Author Jain C.
Title New improved lacZ gene fusion vectors.
Journal Gene
Abstract New plasmid vectors suitable for creating fusions with the lacZ gene have been developed. These vectors represent an improvement over currently available vectors and possess the following features: (1) an undetectable background beta-galactosidase (beta Gal) activity in the absence of fusion, (2) an extended multiple cloning site (MCS), and (3) the ability to conveniently subclone in any one of three translational frames. Medium- and high-copy-number versions of these vectors have been developed.
Volume 133(1)
Pages 99-102
Published 1993-10-29
PII 0378-1119(93)90231-Q
PMID 8224902
MeSH Base Sequence Cloning, Molecular / methods* DNA, Recombinant Genetic Vectors* Lac Operon* Molecular Sequence Data Plasmids Restriction Mapping
IF 2.498
Times Cited 13
Prokaryotes E. coli placZ1 placZ1a placZ2 placZ3 placZY1 placZY1a placZY2 placZY3