論文 - 詳細
| RRC ID | 33378 |
|---|---|
| 著者 | Cha J, Bishai W, Chandrasegaran S. |
| タイトル | New vectors for direct cloning of PCR products. |
| ジャーナル | Gene |
| Abstract |
We describe the construction of two new vectors for direct cloning of polymerase chain reaction (PCR) products. This was done by inserting a synthetic DNA fragment containing two adjacent XcmI sites between the Asp718 and BamHI sites of the M13mp18 and M13mp19 phages. Cleavage of these M13 derivatives with XcmI will result in a linearized vector with a single thymidine nucleotide at the 3' ends. Thus, these vectors would be very useful for direct cloning of PCR-generated products with high efficiency. |
| 巻・号 | 136(1-2) |
| ページ | 369-70 |
| 公開日 | 1993-12-22 |
| DOI | 10.1016/0378-1119(93)90498-r |
| PII | 0378-1119(93)90498-R |
| PMID | 8294034 |
| MeSH | Bacteriophage M13 / genetics Base Sequence Cloning, Molecular / methods* DNA Genetic Vectors* Molecular Sequence Data Polymerase Chain Reaction* |
| IF | 2.984 |
| 引用数 | 35 |
| WOS 分野 | GENETICS & HEREDITY |
| リソース情報 | |
| 原核生物(大腸菌) | pCBC2 pCBC1 |